The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 112 kDa).
ab219772 is capable of detecting both over-expressed and endogenous SIPA1.
GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling SIPA1 with ab219772 at 1.25 µg/ml. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. X40.
Western blot - Anti-SIPA1 antibody - N-terminal (ab219772)
All lanes : Anti-SIPA1 antibody - N-terminal (ab219772) at 1/1250 dilution
Lane 1 : SIPA1 over-expression lysate from Mouse NIH 3T3 cells Lane 2 : Lysate from NIH 3T3 cells mock-transfected with LacZGLB Lane 3 : SIPA1 over-expression lysate from Mouse NIH3T3 cells with immunizing peptide Lane 4 : Lysate from NIH 3T3 cells mock-transfected with LacZGLB with immunizing peptide
Predicted band size: 112 kDa
Lane 2 shows SIPA1 at a significantly reduced level compared to the transfected cells in Lane 1.