Anti-SIPA1 antibody - N-terminal (ab219772)

Overview

  • Product name
    Anti-SIPA1 antibody - N-terminal
    See all SIPA1 primary antibodies
  • Description
    Rabbit polyclonal to SIPA1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse SIPA1 (N terminal).
    Database link: P46062

  • Positive control
    • Human small intestine and tonsil tissues. NIH 3T3 lysate expressing recombinant SIPA1.

Properties

Applications

Our Abpromise guarantee covers the use of ab219772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 112 kDa).

ab219772 is capable of detecting both over-expressed and endogenous SIPA1.

Target

  • Function
    GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
  • Tissue specificity
    Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
  • Sequence similarities
    Contains 1 PDZ (DHR) domain.
    Contains 1 Rap-GAP domain.
  • Cellular localization
    Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTPase activating protein Spa 1 antibody
    • GTPase-activating protein Spa-1 antibody
    • MGC102688 antibody
    • MGC17037 antibody
    • p130 SPA-1 antibody
    • p130 SPA1 antibody
    • Signal induced proliferation associated 1 antibody
    • Signal induced proliferation associated gene 1 antibody
    • Signal induced proliferation associated protein 1 antibody
    • Signal-induced proliferation-associated protein 1 antibody
    • SIPA 1 antibody
    • Sipa-1 antibody
    • Sipa1 antibody
    • SIPA1_HUMAN antibody
    • Spa1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small intestine tissue labeling SIPA1 with ab219772 at 5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling SIPA1 with ab219772 at 5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling SIPA1 with ab219772 at 1.25 µg/ml.  The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. X40.

  • All lanes : Anti-SIPA1 antibody - N-terminal (ab219772) at 1/1250 dilution

    Lane 1 : SIPA1 over-expression lysate from Mouse NIH 3T3 cells
    Lane 2 : Lysate from NIH 3T3 cells mock-transfected with LacZGLB
    Lane 3 : SIPA1 over-expression lysate from Mouse NIH3T3 cells with immunizing peptide
    Lane 4 : Lysate from NIH 3T3 cells mock-transfected with LacZGLB with immunizing peptide

    Predicted band size: 112 kDa



    Lane 2 shows SIPA1 at a significantly reduced level compared to the transfected cells in Lane 1.

References

ab219772 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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