Anti-SIPA1L1 antibody (ab91586)
Key features and details
- Rabbit polyclonal to SIPA1L1
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-SIPA1L1 antibody -
Description
Rabbit polyclonal to SIPA1L1 -
Host species
Rabbit -
Specificity
ab91586 is specific for SIPA1L1 and will not recognize the other SIPA1 family proteins. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Human SIPA1L1 (N terminal). The antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SIPA1L1. It is located within amino acids 40 to 90.
Database link: O43166 -
Positive control
- WB: Rat brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 200 kDa.
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Notes |
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IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 200 kDa. |
Target
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Relevance
SIPA1L1 (Signal-induced proliferation associated-like protein 1) is a member of the SIPA1 family of RapGAPs. SIPA1L1 was initially identified as a binding partner and degradation target of the E6 oncoprotein of high-risk papillomaviruses. Recently, it was discovered that Casein kinase I epsilon (CKIe), a Wnt-regulated kinase that regulates Wnt/b-catenin signaling, also can bind to the carboxy-terminus of SIPA1L1. CKIe phosphorylates SIPA1L1, thereby reducing its stability and alleviating its inhibition of Rap1, a protein required for Wnt8/CKIe-mediated gastrulation during embryogenesis, suggesting SIPA1L1 plays important roles in embryo development as well as control of cell proliferation. -
Cellular localization
Intracellular -
Database links
- Entrez Gene: 246212 Rat
- SwissProt: O35412 Rat
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Alternative names
- DKFZp686G1344 antibody
- E6 targeted protein 1 antibody
- E6TP1 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab91586 has not yet been referenced specifically in any publications.