Anti-SIR2 antibody (ab106169)
Key features and details
- Rabbit polyclonal to SIR2
- Suitable for: ICC, WB
- Reacts with: Trypanosoma brucei
- Isotype: IgG
Overview
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Product name
Anti-SIR2 antibody -
Description
Rabbit polyclonal to SIR2 -
Host species
Rabbit -
Specificity
ab106169 reacts specifically with the 38 kDa protein of Trypanosoma brucei -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Trypanosoma brucei -
Immunogen
Synthetic peptide corresponding to SIR2 (internal sequence).
Database link: Q57V41 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in 200 µl of distilled water. -
Storage instructions
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Purification notes
Lyophilized from 200 µl of serum. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Relevance
SIR2 is a NAD-dependent deacetylase, which participates in a wide range of cellular events including chromosome silencing, chromosome segregation, DNA recombination and the determination of life span. Involved in transcriptional repression of the silent mating-type loci HML and HMR and telomeric silencing via its association with SIR3 and SIR4. Plays a central role in ribosomal DNA (rDNA) silencing via its association with the RENT complex, preventing hyperrecombination, and repressing transcription from foreign promoters, which contributes to extending life span. Probably represses transcription via the formation of heterochromatin structure, which involves the compaction of chromatin fiber into a more condensed form, although this complex in at least one case can still bind euchromatic levels of positive transcription regulators. Although it displays some NAD-dependent histone deacetylase activity on 'Lys-9' and 'Lys-14' of histone H3 and 'Lys-16' of histone H4 in vitro, such activity is unclear in vivo and may not be essential. -
Cellular localization
Nuclear. Nucleolus. -
Alternative names
- Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres antibody
- MAR1 antibody
- NAD dependent histone deacetylase SIR2 antibody
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Protocols
Datasheets and documents
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Datasheet download
References (0)
ab106169 has not yet been referenced specifically in any publications.