Overview

  • Product nameAnti-SIR2 antibody
  • Description
    Rabbit polyclonal to SIR2
  • Specificityab106169 reacts specifically with the 38 kDa protein of Trypanosoma brucei
  • Tested applicationsSuitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Trypanosoma brucei
  • Immunogen

    Synthetic peptides derived from internal regions of the SIR2 protein of Trypanosoma brucei.

Properties

Applications

Our Abpromise guarantee covers the use of ab106169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 38 kDa.
ICC 1/100 - 1/500.

Target

  • RelevanceSIR2 is a NAD-dependent deacetylase, which participates in a wide range of cellular events including chromosome silencing, chromosome segregation, DNA recombination and the determination of life span. Involved in transcriptional repression of the silent mating-type loci HML and HMR and telomeric silencing via its association with SIR3 and SIR4. Plays a central role in ribosomal DNA (rDNA) silencing via its association with the RENT complex, preventing hyperrecombination, and repressing transcription from foreign promoters, which contributes to extending life span. Probably represses transcription via the formation of heterochromatin structure, which involves the compaction of chromatin fiber into a more condensed form, although this complex in at least one case can still bind euchromatic levels of positive transcription regulators. Although it displays some NAD-dependent histone deacetylase activity on 'Lys-9' and 'Lys-14' of histone H3 and 'Lys-16' of histone H4 in vitro, such activity is unclear in vivo and may not be essential.
  • Cellular localizationNuclear. Nucleolus.
  • Alternative names
    • Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres antibody
    • MAR1 antibody
    • NAD dependent histone deacetylase SIR2 antibody
    • Regulatory protein SIR2 antibody
    • Silent information regulator 2 antibody
    • Sir2p antibody
    see all

References for Anti-SIR2 antibody (ab106169)

ab106169 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

For Sir2, we actually carry an antibody that was raise and tested wit T.brucei. It is ab106169:
http://www.abcam.com/SIR2-antibody-ab106169.html

We guarantee it for WB as well as ICC with cultured cel...

Read More

Thank you for your email. I am issuing you the discount codes for testing ab2977 and ab57953 in yeast. Please see below. I have heard back regarding ab106169 and ab51024 - unfortunately, both will not cross react due to lack of sufficient seq...

Read More

Thank you for contacting us. I have checked our catalog and have gathered the following information: 1. STE12: We don't have antibodies to this target protein in our catalog. 2. TEC1(ROC1): ab2977 has 100% homology - and qualifi...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"