The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: 1-2 µg per test is recommended using human blood.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 90 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe protein encoded by the SIRPß1 gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins which are known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPß1 was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Multiple transcript variants encoding different isoforms have been found for this gene.
Cellular localizationCell Membrane; single-pass type I membrane protein
References for Anti-SIRPß1/CD172b antibody [B1D5] (ab55897)
This product has been referenced in:
Liu Y et al. SIRPbeta1 is expressed as a disulfide-linked homodimer in leukocytes and positively regulates neutrophil transepithelial migration. J Biol Chem280:36132-40 (2005).
Read more (PubMed: 16081415) »
Seiffert M et al. Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. Blood97:2741-9 (2001).
Read more (PubMed: 11313266) »