Overview

  • Product nameAnti-SIRPß1/CD172b antibody [B1D5]
    See all SIRPß1/CD172b primary antibodies
  • Description
    Mouse monoclonal [B1D5] to SIRPß1/CD172b
  • SpecificityThe antibody recognizes human SIRPß, but not SIRPa. It reacts with monocytes, granulocytes, CD19+ B-cell pecursors, CD33+ myeloid progenitor cells, and cells transfected with SIRPß1.
  • Tested applicationsSuitable for: IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fusion protein (Human) containing the complete extracellular domain of SIRPß1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purification notesPurified immunoglobulin
  • ClonalityMonoclonal
  • Clone numberB1D5
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
Flow Cyt
  • Application notesFlow Cyt: 1-2 µg per test is recommended using human blood.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 90 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe protein encoded by the SIRPß1 gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins which are known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPß1 was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Multiple transcript variants encoding different isoforms have been found for this gene.
    • Cellular localizationCell Membrane; single-pass type I membrane protein
    • Database links
    • Alternative names
      • CD172 antigen like family member B antibody
      • CD172b antibody
      • CD172b antigen antibody
      • DKFZp686A05192 antibody
      • Signal regulatory protein beta 1 antibody
      • SIRP BETA 1 antibody
      • SIRP beta 1 isoform 3 antibody
      see all

    References for Anti-SIRPß1/CD172b antibody [B1D5] (ab55897)

    This product has been referenced in:
    • Liu Y  et al. SIRPbeta1 is expressed as a disulfide-linked homodimer in leukocytes and positively regulates neutrophil transepithelial migration. J Biol Chem 280:36132-40 (2005). Read more (PubMed: 16081415) »
    • Seiffert M  et al. Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. Blood 97:2741-9 (2001). Read more (PubMed: 11313266) »

    See all 2 Publications for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"