The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells. ab91364-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionImmunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Tissue specificityUbiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
Staining of normal Human peripheral blood cells with Mouse IgG2a k Isotype Control APC (left) or ab95627 (right). Cells in the lymphocyte (green histogram) or granulocyte (blue histogram) gate were used for analysis
References for Anti-SIRP alpha antibody [15-414] (Allophycocyanin) (ab95627)
has not yet been referenced specifically in any publications.
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