Overview

  • Product nameAnti-SIRP alpha antibody
    See all SIRP alpha primary antibodies
  • Description
    Rabbit polyclonal to SIRP alpha
  • SpecificityDetects endogenous levels of total Sirp alpha1 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human SIRP alpha aa 451-500.
    (Peptide available as ab156313)

  • Positive control
    • HepG2 cell extracts; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab53721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionImmunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • Tissue specificityUbiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
  • Sequence similaritiesContains 2 Ig-like C1-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated.
    Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Signal regulatory protein alpha type 1 antibody
    • Bit antibody
    • Brain Ig like molecule with tyrosine based activation motifs antibody
    • Brain Ig-like molecule with tyrosine-based activation motifs antibody
    • Brain immunoglobulin like molecule with tyrosine based activation motifs antibody
    • CD172 antigen like family member A antibody
    • CD172 antigen-like family member A antibody
    • CD172a antibody
    • CD172a antigen antibody
    • Inhibitory receptor SHPS-1 antibody
    • Macrophage fusion receptor antibody
    • MFR antibody
    • MYD 1 antibody
    • Myd 1 antigen antibody
    • MyD-1 antigen antibody
    • p84 antibody
    • Protein tyrosine phosphatase non receptor type substrate 1 antibody
    • PTPNS1 antibody
    • SHP substrate 1 antibody
    • SHPS-1 antibody
    • SHPS1 antibody
    • SHPS1_HUMAN antibody
    • Signal regulatory protein alpha 2 antibody
    • Signal regulatory protein alpha 3 antibody
    • Signal regulatory protein alpha antibody
    • Signal regulatory protein alpha type 2 antibody
    • Signal-regulatory protein alpha-1 antibody
    • Signal-regulatory protein alpha-2 antibody
    • Signal-regulatory protein alpha-3 antibody
    • SIRP antibody
    • Sirp-alpha-1 antibody
    • Sirp-alpha-2 antibody
    • Sirp-alpha-3 antibody
    • SIRPA antibody
    • SIRPalpha antibody
    • SIRPalpha1 antibody
    • SIRPalpha2 antibody
    • SIRPalpha3 antibody
    • Tyrosine phosphatase SHP substrate 1 antibody
    • Tyrosine protein phosphatase non receptor type substrate 1 antibody
    • Tyrosine-protein phosphatase non-receptor type substrate 1 antibody
    see all

Anti-SIRP alpha antibody images

  • All lanes : Anti-SIRP alpha antibody (ab53721) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with immunizing peptide


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Ab53721 at 1/50 dilution, staining paraffin embedded human brain, in the presence (right) and absence (left) of blocking peptide.
  • ICC/IF image of ab53721 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53721, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-SIRP alpha antibody (ab53721)

This product has been referenced in:
  • Ogembo JG  et al. SIRPa/CD172a and FHOD1 are unique markers of littoral cells, a recently evolved major cell population of red pulp of human spleen. J Immunol 188:4496-505 (2012). Read more (PubMed: 22490440) »
  • Mittal R  et al. Escherichia coli K1 promotes the ligation of CD47 with thrombospondin-1 to prevent the maturation of dendritic cells in the pathogenesis of neonatal meningitis. J Immunol 185:2998-3006 (2010). Blocking ; Human . Read more (PubMed: 20675593) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"