Anti-SIRP alpha antibody (ab8120)
Key features and details
- Rabbit polyclonal to SIRP alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SIRP alpha antibody
See all SIRP alpha primary antibodies -
Description
Rabbit polyclonal to SIRP alpha -
Host species
Rabbit -
Specificity
Recognizes SIRPa1, 2 and 3. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Peptide corresponding to amino acids 487 to 503 ofhuman SIRPa1 (1). The sequences of immunogenicpeptide differ from those of mouse, rat and bovine by oneamino acid (1-7).
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Positive control
- THP-1 whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 75-110 kDa.
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ICC/IF |
Use a concentration of 1 µg/ml.
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 75-110 kDa. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
Tissue specificity
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
Sequence similarities
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 140885 Human
- Omim: 602461 Human
- SwissProt: P78324 Human
- Unigene: 581021 Human
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Alternative names
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (10)
ab8120 has been referenced in 10 publications.
- Shui M et al. Anomalous Levels of CD47/Signal Regulatory Protein Alpha in the Hippocampus Lead to Excess Microglial Engulfment in Mouse Model of Perioperative Neurocognitive Disorders. Front Neurosci 16:788675 (2022). PubMed: 35360151
- Ding X et al. Loss of microglial SIRPa promotes synaptic pruning in preclinical models of neurodegeneration. Nat Commun 12:2030 (2021). PubMed: 33795678
- Liu Y et al. Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury. Kidney Dis (Basel) 6:422-433 (2020). PubMed: 33313063
- Li L et al. Signal regulatory protein a protects podocytes through promoting autophagic activity. JCI Insight 5:N/A (2019). PubMed: 30888336
- Elberg G et al. Deletion of SIRPa (signal regulatory protein-a) promotes phagocytic clearance of myelin debris in Wallerian degeneration, axon regeneration, and recovery from nerve injury. J Neuroinflammation 16:277 (2019). PubMed: 31883525
- Whitaker AT et al. Smad4 regulates growth plate matrix production and chondrocyte polarity. Biol Open 6:358-364 (2017). PubMed: 28167493
- Almodóvar-García K et al. ANKRD1 acts as a transcriptional repressor of MMP13 via the AP-1 site. Mol Cell Biol 34:1500-11 (2014). PubMed: 24515436
- Irandoust M et al. Engagement of SIRPa inhibits growth and induces programmed cell death in acute myeloid leukemia cells. PLoS One 8:e52143 (2013). PubMed: 23320069
- Zhao XW et al. CD47-signal regulatory protein-a (SIRPa) interactions form a barrier for antibody-mediated tumor cell destruction. Proc Natl Acad Sci U S A 108:18342-7 (2011). PubMed: 22042861
- van Beek EM et al. Inhibitory regulation of osteoclast bone resorption by signal regulatory protein alpha. FASEB J 23:4081-90 (2009). PubMed: 19667119