Overview

  • Product nameAnti-SIRP alpha antibody
    See all SIRP alpha primary antibodies
  • Description
    Rabbit polyclonal to SIRP alpha
  • SpecificityRecognizes SIRPa1, 2 and 3.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Peptide corresponding to amino acids 487 to 503 ofhuman SIRPa1 (1). The sequences of immunogenicpeptide differ from those of mouse, rat and bovine by oneamino acid (1-7).

  • Positive control
    • THP-1 whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab8120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 75-110 kDa.Can be blocked with SIRP alpha peptide (ab8386).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionImmunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • Tissue specificityUbiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
  • Sequence similaritiesContains 2 Ig-like C1-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated.
    Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Signal regulatory protein alpha type 1 antibody
    • Bit antibody
    • Brain Ig like molecule with tyrosine based activation motifs antibody
    • Brain Ig-like molecule with tyrosine-based activation motifs antibody
    • Brain immunoglobulin like molecule with tyrosine based activation motifs antibody
    • CD172 antigen like family member A antibody
    • CD172 antigen-like family member A antibody
    • CD172a antibody
    • CD172a antigen antibody
    • Inhibitory receptor SHPS-1 antibody
    • Macrophage fusion receptor antibody
    • MFR antibody
    • MYD 1 antibody
    • Myd 1 antigen antibody
    • MyD-1 antigen antibody
    • p84 antibody
    • Protein tyrosine phosphatase non receptor type substrate 1 antibody
    • PTPNS1 antibody
    • SHP substrate 1 antibody
    • SHPS-1 antibody
    • SHPS1 antibody
    • SHPS1_HUMAN antibody
    • Signal regulatory protein alpha 2 antibody
    • Signal regulatory protein alpha 3 antibody
    • Signal regulatory protein alpha antibody
    • Signal regulatory protein alpha type 2 antibody
    • Signal-regulatory protein alpha-1 antibody
    • Signal-regulatory protein alpha-2 antibody
    • Signal-regulatory protein alpha-3 antibody
    • SIRP antibody
    • Sirp-alpha-1 antibody
    • Sirp-alpha-2 antibody
    • Sirp-alpha-3 antibody
    • SIRPA antibody
    • SIRPalpha antibody
    • SIRPalpha1 antibody
    • SIRPalpha2 antibody
    • SIRPalpha3 antibody
    • Tyrosine phosphatase SHP substrate 1 antibody
    • Tyrosine protein phosphatase non receptor type substrate 1 antibody
    • Tyrosine-protein phosphatase non-receptor type substrate 1 antibody
    see all

Anti-SIRP alpha antibody images

  • Anti-SIRP alpha antibody (ab8120) at 1/2000 dilution + THP-1 whole cell lysate
  • ab8120 at 1µg/ml staining SIRP alpha in THP-1 cells by ICC/IF 

References for Anti-SIRP alpha antibody (ab8120)

This product has been referenced in:
  • Zhao XW  et al. CD47-signal regulatory protein-a (SIRPa) interactions form a barrier for antibody-mediated tumor cell destruction. Proc Natl Acad Sci U S A 108:18342-7 (2011). Read more (PubMed: 22042861) »
  • van Beek EM  et al. Inhibitory regulation of osteoclast bone resorption by signal regulatory protein alpha. FASEB J 23:4081-90 (2009). Read more (PubMed: 19667119) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"