Tissue/ cell preparation: Spleen cell homogenate (Rat).
Clone ED9 has been reported to block the interaction of CD172a - CD47. Removal of Sodium Azide is recommended prior to use in functional assays.
Our Abpromise guarantee covers the use of ab34086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
Use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/100 - 1/1000.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.|
ab34086 has not yet been referenced specifically in any publications.