Overview

  • Product nameAnti-SIRP alpha antibody [OX-41] (Biotin)
    See all SIRP alpha primary antibodies
  • Description
    Mouse monoclonal [OX-41] to SIRP alpha (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Peritoneal macrophages (Rat)

  • EpitopeThis clone binds to a different epitope on CD172a than ED 9.

Properties

Applications

Our Abpromise guarantee covers the use of ab33987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Flow Cyt
IHC-P
  • Application notesFlow Cyt: Use neat. Use 10ul to label 106 cells in 100ul.
    IHC-Fr: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution. This product requires protein digestion pre-treatment of paraffin sections, eg.trypsin or pronase.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionImmunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
    • Tissue specificityUbiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
    • Sequence similaritiesContains 2 Ig-like C1-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      N-glycosylated.
      Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Signal regulatory protein alpha type 1 antibody
      • Bit antibody
      • Brain Ig like molecule with tyrosine based activation motifs antibody
      • Brain Ig-like molecule with tyrosine-based activation motifs antibody
      • Brain immunoglobulin like molecule with tyrosine based activation motifs antibody
      • CD172 antigen like family member A antibody
      • CD172 antigen-like family member A antibody
      • CD172a antibody
      • CD172a antigen antibody
      • Inhibitory receptor SHPS-1 antibody
      • Macrophage fusion receptor antibody
      • MFR antibody
      • MYD 1 antibody
      • Myd 1 antigen antibody
      • MyD-1 antigen antibody
      • p84 antibody
      • Protein tyrosine phosphatase non receptor type substrate 1 antibody
      • PTPNS1 antibody
      • SHP substrate 1 antibody
      • SHPS-1 antibody
      • SHPS1 antibody
      • SHPS1_HUMAN antibody
      • Signal regulatory protein alpha 2 antibody
      • Signal regulatory protein alpha 3 antibody
      • Signal regulatory protein alpha antibody
      • Signal regulatory protein alpha type 2 antibody
      • Signal-regulatory protein alpha-1 antibody
      • Signal-regulatory protein alpha-2 antibody
      • Signal-regulatory protein alpha-3 antibody
      • SIRP antibody
      • Sirp-alpha-1 antibody
      • Sirp-alpha-2 antibody
      • Sirp-alpha-3 antibody
      • SIRPA antibody
      • SIRPalpha antibody
      • SIRPalpha1 antibody
      • SIRPalpha2 antibody
      • SIRPalpha3 antibody
      • Tyrosine phosphatase SHP substrate 1 antibody
      • Tyrosine protein phosphatase non receptor type substrate 1 antibody
      • Tyrosine-protein phosphatase non-receptor type substrate 1 antibody
      see all

    References for Anti-SIRP alpha antibody [OX-41] (Biotin) (ab33987)

    This product has been referenced in:
    • Adams S  et al. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J Immunol 161:1853-9 (1998). Read more (PubMed: 9712053) »
    • Robinson AP  et al. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology 57:239-47 (1986). Read more (PubMed: 3512425) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"