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EKPQEVQTSRNVESby a Cysteine residue linker, corresponding to internal sequence amino acids 577-590 of Human SIRT1 (NP_036370.2; NP_001135970.1).
Our Abpromise guarantee covers the use of ab113426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 81 kDa). Primary incubation time: 1 hour. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381).|
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab113426 has not yet been referenced specifically in any publications.