Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Overview
Product name
Anti-SIRT1 antibody
See all SIRT1 products (14) ...
Description
Rabbit polyclonal to SIRT1
Specificity
Anti-SIR2 specifically recognizes mouse SIR2 by immunoblotting and immunofluorescence.
Tested applications
IP, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Dog, Human
Immunogen
Synthetic peptide: ATRQELTDV NYPSDKS (with C-terminal added lysine), corresponding to C terminal amino acids 722-737 of Mouse SIRT1
ATRQELTDV NYPSDKS
Positive control
Mouse 3T3-NIH cells.
General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody is affinity-purified using the immunogen peptide immobilized on agarose.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromosome Structure >> Telomeres
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
-
Western blot - SIRT1 antibody (ab12193)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: 1/1000
ICC/IF: 1/50((determined by indirect immunofluorescent staining of methanol fixed cultured mouse 3T3-NIH cells).)
WB: 1/2000Predicted molecular weight: 80 kDa.((nuclear extract of mouse 3T3-NIH cells). Observed molecular weight is 110 kDa. In some preparations additional lower bands may be detected. )
Target
Function
NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Deacetylates 'Lys-266' of SUV39H1, leading to its activation. Deacetylates 'Lys-26' of HIST1H1E. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Deacetylates H2A. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. Deacetylates APEX1 at 'Lys-6' and 'Lys-7'. Stimulates cellular AP endonuclease activity by promoting the association of APEX1 to XRCC1.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Post-translational
modifications
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.
Cellular localization
Nucleus > PML body. Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane.
Target information above from: UniProt accessionQ96EB6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BA57G10.4 antibody
- BA57G10.4 antibody
- hSIR2 antibody
see all
Database links
- Entrez Gene: 23411 Human
- Entrez Gene: 93759 Mouse
- Omim: 604479 Human
- SwissProt: Q96EB6 Human
- SwissProt: Q923E4 Mouse
- Unigene: 369779 Human
- Unigene: 351459 Mouse
Anti-SIRT1 antibody images:
Western blot - SIRT1 antibody (ab12193)
Anti-SIRT1 antibody (ab12193) at 1/500 dilution (for 1 hour at 21°C) + Whole tissue lysate of mouse jejunum and ileum at 10 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 80 kDa
Observed band size : 80 kDa
Additional bands at : ~45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Blocking Step: 5 µg/mL milk for 16 hours at 4°C.
This image is courtesy of an anonymous Abreview
References for Anti-SIRT1 antibody (ab12193)
This product has been referenced in:
- Campagna Met al. SIRT1 stabilizes PML promoting its sumoylation. Cell Death Differ 18:72-9 (2011). WB, ICC/IF; Mouse.Read more (PubMed: 20577263) »
- Shakibaei Met al. Resveratrol-mediated SIRT-1 Interactions with p300 Modulate Receptor Activator of NF-{kappa}B Ligand (RANKL) Activation of NF-{kappa}B Signaling and Inhibit Osteoclastogenesis in Bone-derived Cells. J Biol Chem 286:11492-505 (2011). WB; Mouse.Read more (PubMed: 21239502) »
See all 7 publications for this product
Publishing research using ab12193? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"