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Western blot - SIRT1 antibody (ab13749)
(enlarge)
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Product Name
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SIRT1 antibody
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See all SIRT1 antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SIRT1
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Immunogen
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Peptide mapping to the carboxy terminal domain of human SIRT1.
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/400. Predicted molecular weight: 82 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
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Relevance
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The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53. There are now five described human homologs of yeast Sir2, which are named SIRT1-5.
SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.
SIRT1 is a HDAC which is important in establishing repressive chromatin structures and plays a role in increasing lifespan. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide, 0.2% Gelatin
Constituents: PBS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SIRT1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SIRT1 antibody images, references, products related to ab13749 and other tools.
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SIRT1 antibody images:
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 Western blot - SIRT1 antibody (ab13749)
All lanes : SIRT1 antibody (ab13749) at 5 µg/ml
Lane 1 : 10 µg Human MCF7 breast cancer whole cell lysate
Lane 2 : 10 µg Human MCF7 breast cancer whole cell lysate, selected for multiple drug resistance with doxorubicin for 72 hours
Secondary
HRP-conjugated Goat anti-Rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
The primary antibody was incubated for 18 hrs at 4°C. The secondary antibody was incubated at room temperature for 30 minutes. All washes were performed using the same buffer (5% PBST/milk) for 3 x 5 minutes. The final washes were in PBST. This image is courtesy of an Abreview submitted by Dr Gerald Davies
See Abreview
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References for SIRT1 antibody (ab13749)
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This product has been used in: (two most recent references)
Borradaile NM & Pickering JG Nicotinamide phosphoribosyltransferase imparts human endothelial cells with extended replicative lifespan and enhanced angiogenic capacity in a high glucose environment. Aging Cell 8:100-12 (2009). WB; Human. PubMed: 19302375
van der Veer E et al. Extension of human cell lifespan by nicotinamide phosphoribosyltransferase. J Biol Chem 282:10841-5 (2007). PubMed: 17307730
If you publish research using ab13749 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to SIRT1
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SIRT1 antibody - more information
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Customer reviews (feedback) regarding SIRT1 antibody |
Customer FAQs regarding SIRT1 antibody |
Protocols for SIRT1 antibody |
Price and availability of products related to SIRT1 antibody |
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SIRT1 antibody - related products:
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SIRT1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SIRT1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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