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Anti-SIRT1 antibody (ab13749)

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Overview

Product name

Anti-SIRT1 antibody
See all SIRT1 products (14) ...

Description

Rabbit polyclonal to SIRT1

Tested applications

WBmore details

Cross reactivity

Reacts with

Cow, Human

Immunogen

Peptide mapping to the carboxy terminal domain of human SIRT1.

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - SIRT1 antibody (ab13749)Western blot - SIRT1 antibody (ab13749) image (enlarge)

  • Western blot - SIRT1 antibody (ab13749)Western blot - SIRT1 antibody (ab13749) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Deacetylates 'Lys-266' of SUV39H1, leading to its activation. Deacetylates 'Lys-26' of HIST1H1E. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Deacetylates H2A. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. Deacetylates APEX1 at 'Lys-6' and 'Lys-7'. Stimulates cellular AP endonuclease activity by promoting the association of APEX1 to XRCC1.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.

Post-translational
modifications

S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.

Cellular localization

Nucleus > PML body. Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane.

Target information above from: UniProt accessionQ96EB6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • BA57G10.4 antibody
  • hSIR2 antibody
  • hSIRT1 antibody
  • MS750 antibody
  • NAD dependent deacetylase SIRT1 antibody
  • NAD dependent deacetylase sirtuin 1 antibody
  • NAD-dependent deacetylase sirtuin-1 antibody
  • OTTHUMP00000198111 antibody
  • OTTHUMP00000198112 antibody
  • SIR2 like 1 antibody
  • SIR2 like protein 1 antibody
  • SIR2-like protein 1 antibody
  • SIR2ALPHA antibody
  • SIR2alpha protein antibody
  • SIR2L1 antibody
  • SIRT 1 antibody
  • Sirt1 antibody
  • SIRT1 Sir2 like proteins (siruitins) type 1 antibody
  • SIRT1: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody
  • SIRT1_HUMAN antibody
  • sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody
  • Sirtuin 1 antibody
  • sirtuin antibody
  • Sirtuin type 1 antibody
see all

Anti-SIRT1 antibody images:

  Western blot - SIRT1 antibody (ab13749)

Western blot - SIRT1 antibody (ab13749)

All lanes : Anti-SIRT1 antibody (ab13749) at 5 µg/ml

Lane 1 : 10 µg Human MCF7 breast cancer whole cell lysate
Lane 2 : 10 µg Human MCF7 breast cancer whole cell lysate, selected for multiple drug resistance with doxorubicin for 72 hours

Secondary
HRP-conjugated Goat anti-Rabbit
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 82 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)


Exposure time : 30 seconds

The primary antibody was incubated for 18 hrs at 4°C.    

The secondary antibody was incubated at room temperature for 30 minutes.

All washes were performed using the same buffer (5% PBST/milk) for 3 x 5 minutes. The final washes were in PBST.

This image is courtesy of an Abreview submitted by Dr Gerald Davies

See Abreview

  Western blot - SIRT1 antibody (ab13749)

Western blot - SIRT1 antibody (ab13749)

Anti-SIRT1 antibody (ab13749) at 1/200 dilution + Human Caco2 epithelial colorectal adenocarcinoma whole cell lysate at 10 µg

Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 82 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)


Exposure time : 30 seconds

Blocking Step: 5% Milk for 16 hours at 4°C

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-SIRT1 antibody (ab13749)

This product has been referenced in:

  • Robers MBet al. Measurement of the cellular deacetylase activity of SIRT1 on p53 via LanthaScreen® technology. Mol Biosyst 7:59-66 (2011). WB; Human.Read more (PubMed: 20931131) »
  • Ho Cet al. SIRT1 markedly extends replicative lifespan if the NAD+ salvage pathway is enhanced. FEBS Lett 583:3081-5 (2009). WB; Human.Read more (PubMed: 19716821) »

See all 5 publications for this product

Publishing research using ab13749? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"