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Products:Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Overview
Product name
Anti-SIRT1 antibody
See all SIRT1 products (14) ...
Description
Rabbit polyclonal to SIRT1
Tested applications
Cross reactivity
Reacts with
Cow, Human
Immunogen
Peptide mapping to the carboxy terminal domain of human SIRT1.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class III / Sir2 class
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/400Predicted molecular weight: 82 kDa.
Target
Function
NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Deacetylates 'Lys-266' of SUV39H1, leading to its activation. Deacetylates 'Lys-26' of HIST1H1E. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Deacetylates H2A. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. Deacetylates APEX1 at 'Lys-6' and 'Lys-7'. Stimulates cellular AP endonuclease activity by promoting the association of APEX1 to XRCC1.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Post-translational
modifications
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.
Cellular localization
Nucleus > PML body. Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane.
Target information above from: UniProt accessionQ96EB6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BA57G10.4 antibody
- hSIR2 antibody
- hSIRT1 antibody
see all
Database links
- Entrez Gene: 23411 Human
- Omim: 604479 Human
- SwissProt: Q96EB6 Human
- Unigene: 369779 Human
Anti-SIRT1 antibody images:
Western blot - SIRT1 antibody (ab13749)
All lanes : Anti-SIRT1 antibody (ab13749) at 5 µg/ml
Lane 1 : 10 µg Human MCF7 breast cancer whole cell lysate
Lane 2 : 10 µg Human MCF7 breast cancer whole cell lysate, selected for multiple drug resistance with doxorubicin for 72 hours
Secondary
HRP-conjugated Goat anti-Rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
The primary antibody was incubated for 18 hrs at 4°C.
The secondary antibody was incubated at room temperature for 30 minutes.
All washes were performed using the same buffer (5% PBST/milk) for 3 x 5 minutes. The final washes were in PBST.
This image is courtesy of an Abreview submitted by Dr Gerald Davies
Western blot - SIRT1 antibody (ab13749)
Anti-SIRT1 antibody (ab13749) at 1/200 dilution + Human Caco2 epithelial colorectal adenocarcinoma whole cell lysate at 10 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Blocking Step: 5% Milk for 16 hours at 4°C
This image is courtesy of an anonymous Abreview
References for Anti-SIRT1 antibody (ab13749)
This product has been referenced in:
- Robers MBet al. Measurement of the cellular deacetylase activity of SIRT1 on p53 via LanthaScreen® technology. Mol Biosyst 7:59-66 (2011). WB; Human.Read more (PubMed: 20931131) »
- Ho Cet al. SIRT1 markedly extends replicative lifespan if the NAD+ salvage pathway is enhanced. FEBS Lett 583:3081-5 (2009). WB; Human.Read more (PubMed: 19716821) »
See all 5 publications for this product
Publishing research using ab13749? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"