Primarily, Abcam’s SIRT2 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT2 inhibitors or activators using recombinant SIRT2 or purified SIRT2.
Applications for this kit include:
1. Screening inhibitors or activators of SIRT2.
2. Detecting the effects of pharmacological agents on SIRT2.
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity. Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain.
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
Phosphorylated at the G2/M transition of the cell cycle.
Cytoplasm > cytoskeleton. Colocalizes with microtubules.