Overview

  • Product name
  • Description
    Rabbit polyclonal to SIRT2
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cynomolgus monkey, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide:

    LEDLVRREHA NI

    conjugated to KLH, corresponding to amino acids 341-352 of Mouse SIR2/ SIRT2

  • Positive control
    • Whole extracts of mouse and rat brain. Extract of COS7 cells expressing recombinant human SIRT2 protein. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab67299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 43 , 38 kDa.
IP Use at an assay dependent dilution. Use at 2 - 5 µg.
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • Function
    NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • Tissue specificity
    Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • Sequence similarities
    Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • Developmental stage
    Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • Post-translational
    modifications
    Phosphorylated at the G2/M transition of the cell cycle.
  • Cellular localization
    Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ35621 antibody
    • FLJ37491 antibody
    • NAD dependent deacetylase sirtuin 2 antibody
    • NAD-dependent deacetylase sirtuin-2 antibody
    • NAD-dependent protein deacetylase sirtuin-2 antibody
    • Regulatory protein SIR2 homolog 2 antibody
    • Silencing information regulator 2 like antibody
    • Silent information regulator 2 antibody
    • SIR2 antibody
    • SIR2 like protein 2 antibody
    • Sir2 related protein type 2 antibody
    • SIR2, S. cerevisiae, homolog-loke 2 antibody
    • SIR2-like protein 2 antibody
    • SIR2L antibody
    • SIR2L2 antibody
    • SIRT2 antibody
    • SIRT2_HUMAN antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
    • Sirtuin 2 antibody
    • Sirtuin type 2 antibody
    see all

Images

  • ab67299 staining SIRT2 in pure rat Schwann cells by ICC/IF (Immunocytochemistry/immunofluorescence) after resveratol (RSV) treatment. Cells were fixed with 4% PFA blocked with 10% Goat serum/ 0.1% Triton x-100/ 0.1% BSA in PBS for  60 minutes at 21°C followed by 10% Goat serum/ 0.5% Triton X-100/ 0.01% BSA in PBS for 15 minutes at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 10% goat serum) overnight at 21°C. An  Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Stimulation  of rSCs with RSV led to an increase of SIRT2 expression in densitometry analysis.

References

This product has been referenced in:
  • Budayeva HG & Cristea IM Human Sirtuin 2 Localization, Transient Interactions, and Impact on the Proteome Point to Its Role in Intracellular Trafficking. Mol Cell Proteomics 15:3107-3125 (2016). Read more (PubMed: 27503897) »
  • Thangjam GS  et al. Hsp90 inhibition suppresses NF-?B transcriptional activation via Sirt-2 in human lung microvascular endothelial cells. Am J Physiol Lung Cell Mol Physiol 310:L964-74 (2016). Read more (PubMed: 27036868) »

See all 6 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
240000 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Primary Neural cells)
Specification
Primary Neural cells
Treatment
Supplemented with and without serum in the culture media.
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Username

Abcam user community

Verified customer

Submitted Sep 12 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate with 0.05% Tween 20 pH 6
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% triton X 100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hypothalamus)
Loading amount
50 µg
Specification
Hypothalamus
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 15 2013

Thank you for contacting Abcam.

I have been able to locate and have attached images for these products as you have requested.

I hope that this information is helpful. Please let me know if you have any questions or there are other wa...

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Thank you for contacting us. SIRT6 The human, mouse and rat proteins are homologous in N terminal and central region of protein. So the antibodies available to these regions will most likely cross-react. ab62739, cross reacts with human, mouse an...

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