Overview

  • Product nameAnti-SIRT2 antibody
    See all SIRT2 primary antibodies
  • Description
    Rabbit polyclonal to SIRT2
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cynomolgus Monkey, Marmoset (common), Orangutan
  • Immunogen

    Synthetic peptide:

    LEDLVRREHA NI

    conjugated to KLH, corresponding to amino acids 341-352 of Mouse SIR2/ SIRT2

  • Positive control
    • Whole extracts of mouse and rat brain. Extract of COS7 cells expressing recombinant human SIRT2 protein. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab67299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 43 , 38 kDa.
IP Use at an assay dependent dilution. Use at 2 - 5 µg.
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • FunctionNAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • Tissue specificityWidely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • Sequence similaritiesBelongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • Developmental stagePeaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • Post-translational
    modifications
    Phosphorylated at the G2/M transition of the cell cycle.
  • Cellular localizationCytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ35621 antibody
    • FLJ37491 antibody
    • NAD dependent deacetylase sirtuin 2 antibody
    • NAD-dependent deacetylase sirtuin-2 antibody
    • NAD-dependent protein deacetylase sirtuin-2 antibody
    • Regulatory protein SIR2 homolog 2 antibody
    • Silencing information regulator 2 like antibody
    • Silent information regulator 2 antibody
    • SIR2 antibody
    • SIR2 like protein 2 antibody
    • Sir2 related protein type 2 antibody
    • SIR2, S. cerevisiae, homolog-loke 2 antibody
    • SIR2-like protein 2 antibody
    • SIR2L antibody
    • SIR2L2 antibody
    • SIRT2 antibody
    • SIRT2_HUMAN antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
    • Sirtuin 2 antibody
    • Sirtuin type 2 antibody
    see all

Anti-SIRT2 antibody images

  • ab67299 staining SIRT2 in pure rat Schwann cells by ICC/IF (Immunocytochemistry/immunofluorescence) after resveratol (RSV) treatment. Cells were fixed with 4% PFA blocked with 10% Goat serum/ 0.1% Triton x-100/ 0.1% BSA in PBS for  60 minutes at 21°C followed by 10% Goat serum/ 0.5% Triton X-100/ 0.01% BSA in PBS for 15 minutes at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 10% goat serum) overnight at 21°C. An  Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Stimulation  of rSCs with RSV led to an increase of SIRT2 expression in densitometry analysis.

References for Anti-SIRT2 antibody (ab67299)

This product has been referenced in:
  • Budayeva HG & Cristea IM Human Sirtuin 2 Localization, Transient Interactions, and Impact on the Proteome Point to Its Role in Intracellular Trafficking. Mol Cell Proteomics 15:3107-3125 (2016). Read more (PubMed: 27503897) »
  • Stettner M  et al. Promoting myelination in an in vitro mouse model of the peripheral nerve system: the effect of wine ingredients. PLoS One 8:e66079 (2013). ICC/IF ; Rat . Read more (PubMed: 23762469) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 240000 cells
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (Primary Neural cells)
Specification Primary Neural cells
Treatment Supplemented with and without serum in the culture media.
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
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Submitted Sep 12 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate with 0.05% Tween 20 pH 6
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% triton X 100
Fixative Paraformaldehyde
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Submitted Nov 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hypothalamus)
Loading amount 50 µg
Specification Hypothalamus
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Feb 15 2013

Thank you for contacting Abcam.

I have been able to locate and have attached images for these products as you have requested.

I hope that this information is helpful. Please let me know if you have any questions or there are other wa...

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Thank you for contacting us. SIRT6 The human, mouse and rat proteins are homologous in N terminal and central region of protein. So the antibodies available to these regions will most likely cross-react. ab62739, cross reacts with human, mouse an...

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