The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Application notesIs unsuitable for IHC-P.
FunctionNAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
Tissue specificityWidely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
Sequence similaritiesBelongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain.
Developmental stagePeaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
Post-translational modificationsPhosphorylated at the G2/M transition of the cell cycle.
Cellular localizationCytoplasm > cytoskeleton. Colocalizes with microtubules.
Flow cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red)(ab51023) or a rabbit IgG (negative)(green).
References for Anti-SIRT2 antibody [EP1668Y] (ab51023)
This product has been referenced in:
Du Y et al. Reduced expression of SIRT2 in serous ovarian carcinoma promotes cell proliferation through disinhibition of CDK4 expression. Mol Med Rep15:1638-1646 (2017).
Read more (PubMed: 28259910) »
van Leeuwen IM et al. Modulation of p53 C-terminal acetylation by mdm2, p14ARF, and cytoplasmic SirT2. Mol Cancer Ther12:471-80 (2013).
Read more (PubMed: 23416275) »