Overview

  • Product nameAnti-SIRT2 antibody [EP1668Y]
    See all SIRT2 primary antibodies
  • Description
    Rabbit monoclonal [EP1668Y] to SIRT2
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminus of human SIR2/ SIRT2.

  • Positive control
    • WB: SKBR-3 cell lysate ICC/IF: PC 12 cells Flow Cyt: HeLa cells
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa).
IP 1/50.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionNAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
    • Tissue specificityWidely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
    • Sequence similaritiesBelongs to the sirtuin family.
      Contains 1 deacetylase sirtuin-type domain.
    • Developmental stagePeaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
    • Post-translational
      modifications
      Phosphorylated at the G2/M transition of the cell cycle.
    • Cellular localizationCytoplasm > cytoskeleton. Colocalizes with microtubules.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ35621 antibody
      • FLJ37491 antibody
      • NAD dependent deacetylase sirtuin 2 antibody
      • NAD-dependent deacetylase sirtuin-2 antibody
      • NAD-dependent protein deacetylase sirtuin-2 antibody
      • Regulatory protein SIR2 homolog 2 antibody
      • Silencing information regulator 2 like antibody
      • Silent information regulator 2 antibody
      • SIR2 antibody
      • SIR2 like protein 2 antibody
      • Sir2 related protein type 2 antibody
      • SIR2, S. cerevisiae, homolog-loke 2 antibody
      • SIR2-like protein 2 antibody
      • SIR2L antibody
      • SIR2L2 antibody
      • SIRT2 antibody
      • SIRT2_HUMAN antibody
      • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
      • Sirtuin 2 antibody
      • Sirtuin type 2 antibody
      see all

    Anti-SIRT2 antibody [EP1668Y] images

    • Immunofluorescence staining of PC-12 cells using ab51023 (green) at a dilution of 1/250. The cells were fixed in 4% PFA and counter stained with an anti-tubulin antibody (red) and DAPI (blue).

    • Anti-SIRT2 antibody [EP1668Y] (ab51023) at 1/1000 dilution + SKBR-3 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 43 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    • Flow cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red)(ab51023) or a rabbit IgG (negative)(green).

    References for Anti-SIRT2 antibody [EP1668Y] (ab51023)

    This product has been referenced in:
    • van Leeuwen IM  et al. Modulation of p53 C-terminal acetylation by mdm2, p14ARF, and cytoplasmic SirT2. Mol Cancer Ther 12:471-80 (2013). Human . Read more (PubMed: 23416275) »
    • Liu Y  et al. Oncogene Ras/phosphatidylinositol 3-kinase signaling targets histone H3 acetylation at lysine 56. J Biol Chem 287:41469-80 (2012). WB . Read more (PubMed: 22982396) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (fibroblast cell)
    Total protein in input 500 µg
    Specification fibroblast cell
    Immuno-precipitation step Protein A/G
    Username

    Abcam user community

    Verified customer

    Submitted Apr 02 2013

    Application Western blot
    Sample Mouse Cell lysate - whole cell (mouse fibroblast)
    Loading amount 50 µg
    Specification mouse fibroblast
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 07 2013

    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116 colon cancer cell line and MEF cell line)
    Loading amount 60 µg
    Specification HCT116 colon cancer cell line and MEF cell line
    Treatment DMSO control and 2 µM SAHA for 24 hrs
    Gel Running Conditions Reduced Denaturing (13%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Christian Marx

    Verified customer

    Submitted Feb 13 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (fibroblast cell)
    Loading amount 50 µg
    Specification fibroblast cell
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 23 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"