WB: Human fetal brain and brain cortex lysates; Mouse brain and cerebral cortex lysates; Rat brain lysate; MCF7 whole cell lysate.
IHC-P: Human, mouse and rat cerebrum tissues.
IHC-Fr: Mouse striatum tissue.
IP: Human fetal brain lysate.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Use at an assay dependent concentration. Detects a band of approximately 36, 43 kDa (predicted molecular weight: 43 kDa).
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain.
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
Phosphorylated at the G2/M transition of the cell cycle.
Cytoplasm > cytoskeleton. Colocalizes with microtubules.