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Abcam’s SIRT3 Activity Assay Kit (Fluorometric) (ab156067) detects SIRT3 activity in lysates.
Primarily, the SIRT3 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT3 inhibitors or activators using crude SIRT3 fraction or purified SIRT3.
Applications for this kit include:
1. Screening inhibitors or activators of SIRT3.
2. Detecting the effects of pharmacological agents on SIRT3.
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.
|Developer||1 x 500µl|
|Fluoro-Deacetylated Peptide (0.2 mM)||1 x 100µl|
|Fluoro-Substrate Peptide (0.2 mM)||1 x 500µl|
|NAD (2 mM)||1 x 500µl|
|Recombinant SIRT3||1 x 200µl|
|SIRT Assay Buffer||2 x 1ml|
|Stop Solution||2 x 1ml|
Our Abpromise guarantee covers the use of ab156067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"