• Product name
  • Description
    Rabbit polyclonal to SIRT5
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 9-286 of Human SIRT5.

  • Positive control
    • PC-3 and U87-MG whole cell lysate; Human hepatoma tissue.



Our Abpromise guarantee covers the use of ab154082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/1000.


  • Function
    NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the sirtuin family. Class III subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Domain
    In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).
  • Cellular localization
    Mitochondrion; Cytoplasm. Mitochondrion and Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NAD dependent deacetylase sirtuin 5 antibody
    • NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody
    • NAD dependent protein deacylase sirtuin 5 mitochondrial antibody
    • NAD-dependent protein deacylase sirtuin-5, mitochondrial antibody
    • Regulatory protein SIR2 homolog 5 antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody
    • Sir2 like 5 antibody
    • SIR2-like protein 5 antibody
    • SIR2L5 antibody
    • SIR5_HUMAN antibody
    • Sirt5 antibody
    • Sirtuin 5 antibody
    • Sirtuin type 5 antibody
    see all


  • All lanes : Anti-SIRT5 antibody (ab154082) at 1/1000 dilution

    Lane 1 : PC-3 whole cell lysate
    Lane 2 : U87-MG whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 33 kDa

    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling SIRT5 with ab154082 at 1/500 dilution.


ab154082 has not yet been referenced specifically in any publications.

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