Overview

  • Product name
  • Description
    Mouse polyclonal to SIRT5
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Rhesus monkey, Orangutan
  • Immunogen

    Full length protein corresponding to amino acids 1-310 of Human SIRT5 (NP_036373.1).

  • Positive control
    • SIRT5 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab168650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.

Target

  • Function
    NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the sirtuin family. Class III subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Domain
    In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).
  • Cellular localization
    Mitochondrion; Cytoplasm. Mitochondrion and Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NAD dependent deacetylase sirtuin 5 antibody
    • NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody
    • NAD dependent protein deacylase sirtuin 5 mitochondrial antibody
    • NAD-dependent protein deacylase sirtuin-5, mitochondrial antibody
    • Regulatory protein SIR2 homolog 5 antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody
    • Sir2 like 5 antibody
    • SIR2-like protein 5 antibody
    • SIR2L5 antibody
    • SIR5_HUMAN antibody
    • Sirt5 antibody
    • Sirtuin 5 antibody
    • Sirtuin type 5 antibody
    see all

Images

  • All lanes : Anti-SIRT5 antibody (ab168650) at 1 µg/ml

    Lane 1 : SIRT5 transfected 293T cell lysate

    Lane 2 : Non-transfected 293T cell lysate


    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP
    at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa

References

ab168650 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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