Overview

  • Product nameAnti-SIRT5 antibody
    See all SIRT5 primary antibodies
  • Description
    Rabbit polyclonal to SIRT5
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 179-228 (ICPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLD P) of Human SIRT5 (NP_036373).

  • Positive control
    • human fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionNAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the sirtuin family. Class III subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • DomainIn contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).
  • Cellular localizationMitochondrion; Cytoplasm. Mitochondrion and Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NAD dependent deacetylase sirtuin 5 antibody
    • NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody
    • NAD dependent protein deacylase sirtuin 5 mitochondrial antibody
    • NAD-dependent protein deacylase sirtuin-5, mitochondrial antibody
    • Regulatory protein SIR2 homolog 5 antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody
    • Sir2 like 5 antibody
    • SIR2-like protein 5 antibody
    • SIR2L5 antibody
    • SIR5_HUMAN antibody
    • Sirt5 antibody
    • Sirtuin 5 antibody
    • Sirtuin type 5 antibody
    see all

Anti-SIRT5 antibody images

  • Anti-SIRT5 antibody (ab86274) at 1 µg/ml (5% skim milk/ PBS buffer) + fetal heart lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine labelling SIRT5 with ab86274.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human vessels labelling SIRT5 with ab86274.

References for Anti-SIRT5 antibody (ab86274)

ab86274 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"