Overview

  • Product nameAnti-SIRT5 antibody [EPR5411]
    See all SIRT5 primary antibodies
  • Description
    Rabbit monoclonal [EPR5411] to SIRT5
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of Human SIRT5 (UniProt Q9NXA8).

  • Positive control
    • Fetal liver, HeLa and A431 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/10000.

  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionNAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the sirtuin family. Class III subfamily.
      Contains 1 deacetylase sirtuin-type domain.
    • DomainIn contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).
    • Cellular localizationMitochondrion; Cytoplasm. Mitochondrion and Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • NAD dependent deacetylase sirtuin 5 antibody
      • NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody
      • NAD dependent protein deacylase sirtuin 5 mitochondrial antibody
      • NAD-dependent protein deacylase sirtuin-5, mitochondrial antibody
      • Regulatory protein SIR2 homolog 5 antibody
      • Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody
      • Sir2 like 5 antibody
      • SIR2-like protein 5 antibody
      • SIR2L5 antibody
      • SIR5_HUMAN antibody
      • Sirt5 antibody
      • Sirtuin 5 antibody
      • Sirtuin type 5 antibody
      see all

    Anti-SIRT5 antibody [EPR5411] images

    • Anti-SIRT5 antibody [EPR5411] (ab108968) at 1/5000 dilution + Human Fetal liver lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

      Predicted band size : 34 kDa
    • All lanes : Anti-SIRT5 antibody [EPR5411] (ab108968) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal liver cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit. at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-SIRT5 antibody [EPR5411] (ab108968)

    ab108968 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab108968.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"