Overview

  • Product nameAnti-SIRT6 antibody
    See all SIRT6 primary antibodies
  • Description
    Rabbit polyclonal to SIRT6
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to residues in Human SIRT6 (NP_057623.1).

  • Positive control
    • Human testis tissue; HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab118487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 39 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionNAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
  • Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Cellular localizationNucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810449N18Rik antibody
    • AI043036 antibody
    • Mono ADP ribosyltransferase sirtuin 6 antibody
    • NAD-dependent protein deacetylase sirtuin-6 antibody
    • Regulatory protein SIR2 homolog 6 antibody
    • Regulatory protein SIR2 homolog antibody
    • SIR2 like 6 antibody
    • SIR2 like protein 6 antibody
    • Sir2 related protein type 6 antibody
    • SIR2-like protein 6 antibody
    • SIR2L6 antibody
    • SIR6_HUMAN antibody
    • SIRT 6 antibody
    • SIRT6 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
    • Sirtuin 6 antibody
    • Sirtuin type 6 antibody
    • Sirtuin6 antibody
    see all

Anti-SIRT6 antibody images

  • Anti-SIRT6 antibody (ab118487) + U2OS cell lysate

    Predicted band size : 39 kDa
  • ab118487 at 10µg/ml staining SIRT6 in Formalin-fixed, Paraffin-embedded Human testis tissue by immunohistochemistry.
  • Anti-SIRT6 antibody (ab118487) at 1.25 µg/ml + HepG2 cell lysate

    Predicted band size : 39 kDa

References for Anti-SIRT6 antibody (ab118487)

ab118487 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that the SIRT6 antibody (ab118487) specifically recognizes the SIRT6 protein. The reason why 2 bands are detected on WB is due to the fact that the SIRT6 protein has 2 isoforms: one of 39kDa and the other one of 36kDa. The difference on t...

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Thank you for calling Abcam earlier today.

Please find the protocol attached that was used for antibody ab118487 for IHC with formalin-fixed paraffin-embedded tissues.

I was wondering how it compared to the protocol that you were usin...

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