Overview

  • Product nameAnti-SIRT6 antibody
    See all SIRT6 primary antibodies
  • Description
    Rabbit polyclonal to SIRT6
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    SPKRERPTSPAPHRPPKRV

    conjugated to KLH by a Cysteine residue linker, corresponding to C terminal amino acids 330-348 of Human SIRT6

  • Positive control
    • Extracts from human U87 cells, HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Use at a concentration of 0.5 - 1.0 ug/ml, using whole extracts of human U87 cells and at a concentration of 2.5 - 5.0 ug/ml, using human HeLa cells. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ICC/IF Use a concentration of 2.5 - 5 µg/ml.

Target

  • FunctionNAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
  • Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Cellular localizationNucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810449N18Rik antibody
    • AI043036 antibody
    • Mono ADP ribosyltransferase sirtuin 6 antibody
    • NAD-dependent protein deacetylase sirtuin-6 antibody
    • Regulatory protein SIR2 homolog 6 antibody
    • Regulatory protein SIR2 homolog antibody
    • SIR2 like 6 antibody
    • SIR2 like protein 6 antibody
    • Sir2 related protein type 6 antibody
    • SIR2-like protein 6 antibody
    • SIR2L6 antibody
    • SIR6_HUMAN antibody
    • SIRT 6 antibody
    • SIRT6 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
    • Sirtuin 6 antibody
    • Sirtuin type 6 antibody
    • Sirtuin6 antibody
    see all

Anti-SIRT6 antibody images

  • Immunocytochemistry/ Immunofluorescence analysis of human HeLa cells fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. SIRT6 was labeled with 5 µg/mL ab62738 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate (A) or with DAPI (B).

  • Lane 1 : Anti-SIRT6 antibody (ab62738) at 0.5 µg/ml
    Lane 2 : Anti-SIRT6 antibody (ab62738) at 1 µg/ml
    Lanes 3 - 4 : Anti-SIRT6 antibody (ab62738) at 2 µg/ml

    Lane 1 : Human U87 whole cell extract
    Lane 2 : Human U87 whole cell extract
    Lane 3 : Human U87 whole cell extract
    Lane 4 : Human U87 whole cell extract with immunizing peptide

    Secondary
    Goat Anti-Rabbit IgG
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 39 kDa

References for Anti-SIRT6 antibody (ab62738)

ab62738 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (smooth muscle cells)
Loading amount 10 µg
Specification smooth muscle cells
Treatment senescence
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lung)
Loading amount 100000 cells
Specification Lung
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"