Overview

  • Product name
  • Description
    Rabbit polyclonal to SIRT6
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide mapping to a region between residue 305 and 355 of Human SIRT6 (NP_057623.1)

  • Positive control
    • Whole cell lysate from HeLa or 293T cells

Properties

Applications

Our Abpromise guarantee covers the use of ab88494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
  • Sequence similarities
    Belongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Cellular localization
    Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810449N18Rik antibody
    • AI043036 antibody
    • Mono ADP ribosyltransferase sirtuin 6 antibody
    • NAD-dependent protein deacetylase sirtuin-6 antibody
    • Regulatory protein SIR2 homolog 6 antibody
    • Regulatory protein SIR2 homolog antibody
    • SIR2 like 6 antibody
    • SIR2 like protein 6 antibody
    • Sir2 related protein type 6 antibody
    • SIR2-like protein 6 antibody
    • SIR2L6 antibody
    • SIR6_HUMAN antibody
    • SIRT 6 antibody
    • SIRT6 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
    • Sirtuin 6 antibody
    • Sirtuin type 6 antibody
    • Sirtuin6 antibody
    see all

Anti-SIRT6 antibody images

  • All lanes : Anti-SIRT6 antibody (ab88494) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 39 kDa


    Exposure time : 3 minutes
  • 3ug ab88484 were used to immunoprecipitate 1mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88484 at 1ug/ml.
    Detection: chemoluminescence with exposure time of 30 seconds.

References for Anti-SIRT6 antibody (ab88494)

This product has been referenced in:

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma)
Gel Running Conditions
Reduced Denaturing
Loading amount
500000 cells
Specification
melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Apr 23 2015

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (293)
Specification
293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 24 2013

Thank you for your question.

I hope I can clarify the protocol:

Step 5. is a repetition of step 4.: this provides you the pellet for furtheruse.
Step 6. of the protocol: Re-suspend the pellet in 0.2 N HCl at a density of 4x107 ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoblastoid cell line)
Loading amount
20 µg
Specification
lymphoblastoid cell line
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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