The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). 2-4 ug/mL is recommended using whole extracts of mouse 3T3 cells. Use at 1-2 ug/mL with whole extracts of human U87 cells. Detects a band of approximately 37 kDa (predicted molecular weight: 39 (human), 37 mouse kDa (mouse)).
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 22196736
1/200. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionNAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain.
Cellular localizationNucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
NAD-dependent protein deacetylase sirtuin-6 antibody
Regulatory protein SIR2 homolog 6 antibody
Regulatory protein SIR2 homolog antibody
SIR2 like 6 antibody
SIR2 like protein 6 antibody
Sir2 related protein type 6 antibody
SIR2-like protein 6 antibody
SIRT 6 antibody
Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
Sirtuin 6 antibody
Sirtuin type 6 antibody
Anti-SIRT6 antibody - ChIP Grade images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT6 antibody - ChIP Grade (ab62739)This image is courtesy of an anonymous Abreview
ab62739 staining SIRT6 in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Lane 1 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 0.5 µg/ml Lane 2 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 1 µg/ml Lanes 3 - 4 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 2 µg/ml
Lane 1 : Mouse 3T3 whole cell extract Lane 2 : Mouse 3T3 whole cell extract Lane 3 : Mouse 3T3 whole cell extract Lane 4 : Mouse 3T3 whole cell extract with immunizing peptide
Secondary Goat Anti-Rabbit IgG, developed using the ECL technique
Predicted band size : 37 kDa Observed band size : 37 kDa
References for Anti-SIRT6 antibody - ChIP Grade (ab62739)
This product has been referenced in:
Kim TH et al. Psammaplin A induces Sirtuin 1-dependent autophagic cell death in doxorubicin-resistant MCF-7/adr human breast cancer cells and xenografts. Biochim Biophys Acta1850:401-10 (2015).
Read more (PubMed: 25445714) »
Kugel S et al.
Identification of and Molecular Basis for SIRT6 Loss-of-Function Point Mutations in Cancer.
Cell Rep13:479-88 (2015).
Read more (PubMed: 26456828) »