Overview

  • Product nameAnti-SIRT6 antibody - ChIP Grade
    See all SIRT6 primary antibodies
  • Description
    Rabbit polyclonal to SIRT6 - ChIP Grade
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, CHIPseq, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    GLPEIFDPPEELERK

    conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 19-33 of Human SIRT6

  • Positive control
    • Extracts of human U87 cells, extracts of mouse 3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). 2-4 ug/mL is recommended using whole extracts of mouse 3T3 cells. Use at 1-2 ug/mL with whole extracts of human U87 cells. Detects a band of approximately 37 kDa (predicted molecular weight: 39 (human), 37 mouse kDa (mouse)).
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200.
CHIPseq Use at an assay dependent concentration. PubMed: 22196736
Flow Cyt 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionNAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
  • Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Cellular localizationNucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810449N18Rik antibody
    • AI043036 antibody
    • Mono ADP ribosyltransferase sirtuin 6 antibody
    • NAD-dependent protein deacetylase sirtuin-6 antibody
    • Regulatory protein SIR2 homolog 6 antibody
    • Regulatory protein SIR2 homolog antibody
    • SIR2 like 6 antibody
    • SIR2 like protein 6 antibody
    • Sir2 related protein type 6 antibody
    • SIR2-like protein 6 antibody
    • SIR2L6 antibody
    • SIR6_HUMAN antibody
    • SIRT 6 antibody
    • SIRT6 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
    • Sirtuin 6 antibody
    • Sirtuin type 6 antibody
    • Sirtuin6 antibody
    see all

Anti-SIRT6 antibody - ChIP Grade images

  • ab62739 staining SIRT6 in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Lane 1 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 0.5 µg/ml
    Lane 2 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 1 µg/ml
    Lanes 3 - 4 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 2 µg/ml

    Lane 1 : Mouse 3T3 whole cell extract
    Lane 2 : Mouse 3T3 whole cell extract
    Lane 3 : Mouse 3T3 whole cell extract
    Lane 4 : Mouse 3T3 whole cell extract with immunizing peptide

    Secondary
    Goat Anti-Rabbit IgG,
    Developed using the ECL technique

    Predicted band size : 37 kDa
    Observed band size : 37 kDa

References for Anti-SIRT6 antibody - ChIP Grade (ab62739)

This product has been referenced in:
  • Zhang ZQ  et al. Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice. Sci Rep 6:23912 (2016). ChIP, WB . Read more (PubMed: 27045575) »
  • Kim TH  et al. Psammaplin A induces Sirtuin 1-dependent autophagic cell death in doxorubicin-resistant MCF-7/adr human breast cancer cells and xenografts. Biochim Biophys Acta 1850:401-10 (2015). Read more (PubMed: 25445714) »

See all 16 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Hepatocyte)
Gel Running Conditions Reduced Denaturing (gradient)
Loading amount 10 µg
Specification Hepatocyte
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted Sep 06 2016

Application ChIP
Detection step Real-time PCR
Sample Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification primary hepatoctyes
Negative control Rabbit IgG
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control H3k9ac
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Submitted Dec 04 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 29°C
Sample Mouse Cell (GL261-mouse glioma)
Specification GL261-mouse glioma
Permeabilization Yes - 0.3% TritonX-100 in 1X TBS
Fixative Paraformaldehyde
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Submitted Dec 03 2014

Application Western blot
Loading amount 240000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (Primary Neural cells)
Specification Primary Neural cells
Treatment Supplemented with and without serum in the culture media.
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
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Submitted Sep 16 2014

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 07 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Permeabilization No
Fixative Paraformaldehyde
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Submitted Jun 28 2014

Application Immunoprecipitation
Immuno-precipitation step Protein A
Sample Mouse Cell lysate - whole cell (55.1C cell)
Specification 55.1C cell
Total protein in input 30 µg
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Submitted Mar 14 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Mouse Cell (primary hepatocytes)
Specification primary hepatocytes
Permeabilization No
Fixative Paraformaldehyde
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Submitted Jan 23 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification primary hepatoctyes
Blocking step I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
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Submitted Oct 29 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Jun 07 2013

1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"