The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). 2-4 ug/mL is recommended using whole extracts of mouse 3T3 cells. Use at 1-2 ug/mL with whole extracts of human U87 cells. Detects a band of approximately 37 kDa (predicted molecular weight: 39 (human), 37 mouse kDa (mouse)).
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 22196736
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionNAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain.
Cellular localizationNucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
NAD-dependent protein deacetylase sirtuin-6 antibody
Regulatory protein SIR2 homolog 6 antibody
Regulatory protein SIR2 homolog antibody
SIR2 like 6 antibody
SIR2 like protein 6 antibody
Sir2 related protein type 6 antibody
SIR2-like protein 6 antibody
SIRT 6 antibody
Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
Sirtuin 6 antibody
Sirtuin type 6 antibody
Anti-SIRT6 antibody - ChIP Grade images
ChIP - Anti-SIRT6 antibody - ChIP Grade (ab62739)This image is courtesy of an anonymous abreview.
ChIP analysis using ab62739 binding SIRT6 in mouse primary hepatoctyes. Cells were cross-linked for 8 minutes with 1% formaldehyde. Samples were incubated with Anti-SIRT6 antibody - ChIP Grade (ab62739) (1/200) for 20 hours at 4°C. Protein binding was detected using real-time PCR.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT6 antibody - ChIP Grade (ab62739)This image is courtesy of an anonymous Abreview
ab62739 staining SIRT6 in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling SIRT6 with ab62739. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 2.5 μg/mL Anti-SIRT6 antibody - ChIP Grade (ab62739). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate.
Western blot - Anti-SIRT6 antibody - ChIP Grade (ab62739)
Lane 1 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 0.5 µg/ml Lane 2 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 1 µg/ml Lanes 3 - 4 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 2 µg/ml
Lane 1 : Mouse 3T3 whole cell extract Lane 2 : Mouse 3T3 whole cell extract Lane 3 : Mouse 3T3 whole cell extract Lane 4 : Mouse 3T3 whole cell extract with immunizing peptide
Secondary Goat Anti-Rabbit IgG, Developed using the ECL technique
Predicted band size : 37 kDa Observed band size : 37 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SIRT6 antibody - ChIP Grade (ab62739)This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence analysis of mouse primary hepatocytes labeling SIRT6 with ab62739 at 1/200 dilution. The cells were fixed with paraformaldehyde, followed by blocking with 3% BSA for 2 hours at 20°C. A polyclonal goat anti-rabbit IgG Alexa Fluor® 488 secondary antibody was used at 1/10000 dilution.
References for Anti-SIRT6 antibody - ChIP Grade (ab62739)
This product has been referenced in:
Zhang ZQ et al. Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice. Sci Rep6:23912 (2016).
Read more (PubMed: 27045575) »
Kim TH et al. Psammaplin A induces Sirtuin 1-dependent autophagic cell death in doxorubicin-resistant MCF-7/adr human breast cancer cells and xenografts. Biochim Biophys Acta1850:401-10 (2015).
Read more (PubMed: 25445714) »