Overview

  • Product name
    Anti-SIRT6 antibody - ChIP Grade
    See all SIRT6 primary antibodies
  • Description
    Rabbit polyclonal to SIRT6 - ChIP Grade
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, CHIPseq, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    GLPEIFDPPEELERK

    conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 19-33 of Human SIRT6

  • Positive control
    • Extracts of human U87 cells, extracts of mouse 3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). 2-4 ug/mL is recommended using whole extracts of mouse 3T3 cells. Use at 1-2 ug/mL with whole extracts of human U87 cells. Detects a band of approximately 37 kDa (predicted molecular weight: 39 (human), 37 mouse kDa (mouse)).
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200.
CHIPseq Use at an assay dependent concentration. PubMed: 22196736
Flow Cyt 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
  • Sequence similarities
    Belongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Cellular localization
    Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810449N18Rik antibody
    • AI043036 antibody
    • Mono ADP ribosyltransferase sirtuin 6 antibody
    • NAD-dependent protein deacetylase sirtuin-6 antibody
    • Regulatory protein SIR2 homolog 6 antibody
    • Regulatory protein SIR2 homolog antibody
    • SIR2 like 6 antibody
    • SIR2 like protein 6 antibody
    • Sir2 related protein type 6 antibody
    • SIR2-like protein 6 antibody
    • SIR2L6 antibody
    • SIR6_HUMAN antibody
    • SIRT 6 antibody
    • SIRT6 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
    • Sirtuin 6 antibody
    • Sirtuin type 6 antibody
    • Sirtuin6 antibody
    see all

Images

  • ChIP analysis using ab62739 binding SIRT6 in mouse primary hepatoctyes. Cells were cross-linked for 8 minutes with 1% formaldehyde. Samples were incubated with Anti-SIRT6 antibody - ChIP Grade (ab62739) (1/200) for 20 hours at 4°C. Protein binding was detected using real-time PCR.

    See Abreview

  • ab62739 staining SIRT6 in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling SIRT6 with ab62739. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2.5 μg/mL Anti-SIRT6 antibody - ChIP Grade (ab62739). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

  • Lane 1 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 0.5 µg/ml
    Lane 2 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 1 µg/ml
    Lanes 3 - 4 : Anti-SIRT6 antibody - ChIP Grade (ab62739) at 2 µg/ml

    Lane 1 : Mouse 3T3 whole cell extract
    Lane 2 : Mouse 3T3 whole cell extract
    Lane 3 : Mouse 3T3 whole cell extract
    Lane 4 : Mouse 3T3 whole cell extract with immunizing peptide

    Secondary
    Goat Anti-Rabbit IgG,
    Developed using the ECL technique

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • Immunocytochemistry/ Immunofluorescence analysis of mouse primary hepatocytes labeling SIRT6 with ab62739 at 1/200 dilution. The cells were fixed with paraformaldehyde, followed by blocking with 3% BSA for 2 hours at 20°C. A polyclonal goat anti-rabbit IgG Alexa Fluor® 488 secondary antibody was used at 1/10000 dilution.

    See Abreview

References

This product has been referenced in:
  • Diani-Moore S  et al. NAD(+) loss, a new player in AhR biology: prevention of thymus atrophy and hepatosteatosis by NAD(+) repletion. Sci Rep 7:2268 (2017). WB . Read more (PubMed: 28536482) »
  • Ioris RM  et al. SIRT6 Suppresses Cancer Stem-like Capacity in Tumors with PI3K Activation Independently of Its Deacetylase Activity. Cell Rep 18:1858-1868 (2017). ChIP . Read more (PubMed: 28228253) »

See all 20 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
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Submitted Jul 17 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepatocyte)
Gel Running Conditions
Reduced Denaturing (gradient)
Loading amount
10 µg
Specification
Hepatocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted Sep 06 2016

Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Negative control
Rabbit IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
H3k9ac
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Submitted Dec 04 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 29°C
Sample
Mouse Cell (GL261-mouse glioma)
Specification
GL261-mouse glioma
Permeabilization
Yes - 0.3% TritonX-100 in 1X TBS
Fixative
Paraformaldehyde
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Submitted Dec 03 2014

Application
Western blot
Loading amount
240000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (Primary Neural cells)
Specification
Primary Neural cells
Treatment
Supplemented with and without serum in the culture media.
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
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Submitted Sep 16 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 07 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Permeabilization
No
Fixative
Paraformaldehyde
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Submitted Jun 28 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Mouse Cell lysate - whole cell (55.1C cell)
Specification
55.1C cell
Total protein in input
30 µg
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Submitted Mar 14 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Mouse Cell (primary hepatocytes)
Specification
primary hepatocytes
Permeabilization
No
Fixative
Paraformaldehyde
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Submitted Jan 23 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
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Submitted Oct 29 2013

1-10 of 24 Abreviews or Q&A

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