Anti-SIRT7 antibody [1E2B2] (ab234088)
Key features and details
- Mouse monoclonal [1E2B2] to SIRT7
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-SIRT7 antibody [1E2B2]
See all SIRT7 primary antibodies -
Description
Mouse monoclonal [1E2B2] to SIRT7 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human SIRT7 aa 1-105. Expressed in E. coli.
Database link: Q9NRC8 -
Positive control
- WB: SIRT7 (amino acids: 1-105)-hIgGFc transfected HEK-293 cell lysate; Human recombinant SIRT7 (amino acids: 1-105) protein. Flow Cytometry: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1E2B2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/100 - 1/500.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/100 - 1/500. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation. -
Sequence similarities
Belongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain. -
Post-translational
modificationsPhosphorylated during mitosis. -
Cellular localization
Cytoplasm. Nucleus, nucleolus. Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 51547 Human
- Omim: 606212 Human
- SwissProt: Q9NRC8 Human
- Unigene: 514636 Human
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Alternative names
- NAD dependent deacetylase sirtuin 7 antibody
- NAD dependent protein deacetylase sirtuin 7 antibody
- NAD-dependent protein deacetylase sirtuin-7 antibody
see all
Images
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Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SIRT7 with ab234088 at 1/200 dilution (green) compared to a negative control (red).
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All lanes : Anti-SIRT7 antibody [1E2B2] (ab234088) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : SIRT7 (amino acids: 1-105)-hIgGFc transfected HEK-293 cell lysate -
Anti-SIRT7 antibody [1E2B2] (ab234088) at 1/100 dilution + Human recombinant SIRT7 (amino acids: 1-105) protein
Expected recombinant MW is 38 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab234088 has not yet been referenced specifically in any publications.