Overview

  • Product name
  • Description
    Rabbit polyclonal to SIRT7
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide:

    ILRKAAAERSAEEGRLL

    conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 35-51 of Human SIRT7

  • Positive control
    • Extracts of HEK-293T cells expressing human SIRT7, HEK-293T cells.

Applications

Our Abpromise guarantee covers the use of ab62748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 2 - 4 ug/ml.
    IP: Use at 2-4 ug.
    WB: Use at a concentration of 1 - 2 ug/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.
    • Sequence similarities
      Belongs to the sirtuin family. Class IV subfamily.
      Contains 1 deacetylase sirtuin-type domain.
    • Post-translational
      modifications
      Phosphorylated during mitosis.
    • Cellular localization
      Cytoplasm. Nucleus, nucleolus. Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • NAD dependent deacetylase sirtuin 7 antibody
      • NAD dependent protein deacetylase sirtuin 7 antibody
      • NAD-dependent protein deacetylase sirtuin-7 antibody
      • Regulatory protein SIR2 homolog 7 antibody
      • Silent mating type information regulation 2 homolog antibody
      • Silent mating type information regulation 2 S.cerevisiae homolog 7 antibody
      • SIR2 L7 antibody
      • SIR2 like protein 7 antibody
      • Sir2 related protein type 7 antibody
      • SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7 antibody
      • SIR2-like protein 7 antibody
      • SIR2L 7 antibody
      • SIR2L7 antibody
      • SIR7_HUMAN antibody
      • SIRT 7 antibody
      • SIRT7 antibody
      • Sirtuin 7 antibody
      • Sirtuin type 7 antibody
      • Sirtuin7 antibody
      see all

    Anti-SIRT7 antibody images

    • Human HEK-293T cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton-X-100. Cells were stained with 2.5 µg/mL Anti-SIRT7 (ab62748) and developed with Goat Anti-Rabbit IgG, Cy3 conjugate (A), or stained with DAPI (B).
    • ab62748 was used to immunoprecipitate SIRT7 from HEK-293T cells expressing recombinant human SIRT7-V5. Detection antibody: Monoclonal Anti-V5 antibody. Lanes 1: 2 µg Antibody 2: 4 µg Antibody 3: Negative control (without primary antibody)

    References for Anti-SIRT7 antibody (ab62748)

    This product has been referenced in:
    • Dass RA  et al. Wnt5a Signals through DVL1 to Repress Ribosomal DNA Transcription by RNA Polymerase I. PLoS Genet 12:e1006217 (2016). IHC-P . Read more (PubMed: 27500936) »

    See 1 Publication for this product

    Product Wall

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain, Kidney, Heart)
    Gel Running Conditions
    Reduced Denaturing (10% Gel)
    Loading amount
    50 µg
    Specification
    Brain, Kidney, Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Mona Dvir-Ginzberg

    Verified customer

    Submitted Feb 28 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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