Our Abpromise guarantee covers the use of ab78977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 45 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
ab78977 staining SIRT7 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% triton X-100 and blocked with 3% BSA for 2 hours at 25°C; antigen retrieval was by heat mediation in 0.1M citrate buffer containing 0.05% triton X-100. Samples were incubated with primary antibody (1/1000 in 1% BSA containing 0.1% triton X-100) for 16 hours at 4°C. A Cy3 ®-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.