Overview

  • Product nameAnti-SIRT7 antibody
    See all SIRT7 primary antibodies
  • Description
    Rabbit polyclonal to SIRT7
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    A mixture of synthetic peptides corresponding to amino acids 35-51 and 361-377 of Human SIRT7.

  • Positive control
    • Kidney tissue.

Associated products

Applications

Our Abpromise guarantee covers the use of ab78977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionNAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.
  • Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Post-translational
    modifications
    Phosphorylated during mitosis.
  • Cellular localizationCytoplasm. Nucleus, nucleolus. Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • NAD dependent deacetylase sirtuin 7 antibody
    • NAD dependent protein deacetylase sirtuin 7 antibody
    • NAD-dependent protein deacetylase sirtuin-7 antibody
    • Regulatory protein SIR2 homolog 7 antibody
    • Silent mating type information regulation 2 homolog antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 7 antibody
    • SIR2 L7 antibody
    • SIR2 like protein 7 antibody
    • Sir2 related protein type 7 antibody
    • SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7 antibody
    • SIR2-like protein 7 antibody
    • SIR2L 7 antibody
    • SIR2L7 antibody
    • SIR7_HUMAN antibody
    • SIRT 7 antibody
    • SIRT7 antibody
    • Sirtuin 7 antibody
    • Sirtuin type 7 antibody
    • Sirtuin7 antibody
    see all

Anti-SIRT7 antibody images

  • ab78977 staining SIRT7 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% triton X-100 and blocked with 3% BSA for 2 hours at 25°C; antigen retrieval was by heat mediation in 0.1M citrate buffer containing 0.05% triton X-100. Samples were incubated with primary antibody (1/1000 in 1% BSA containing 0.1% triton X-100) for 16 hours at 4°C. A Cy3 ®-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab78977 at 5µg/ml staining SIRT7 in kidney tissue.

References for Anti-SIRT7 antibody (ab78977)

This product has been referenced in:
  • McGlynn LM  et al. SIRT3 & SIRT7: Potential Novel Biomarkers for Determining Outcome in Pancreatic Cancer Patients. PLoS One 10:e0131344 (2015). IHC ; Human . Read more (PubMed: 26121130) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 240000 cells
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (Primary Neural cells)
Specification Primary Neural cells
Treatment Supplemented with and without serum in the culture media.
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
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Submitted Sep 12 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.1M Citrate buffer containing 0.05% tritonX100
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% triton X100
Fixative Paraformaldehyde
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Submitted Oct 29 2013

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Mar 26 2013

Thanks again for contacting us with your question, and for your patience. The peptide for ab49173 is now available on our website as ab123888- http://www.abcam.com/SIRT5-peptide-ab123888.html Unfortunately the peptide for ab78977 is not available for s...

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Thank you for contacting us with your question. We do have the blocking peptide for the SIRT5 antibody ab49173, but it isn't on our website at this point so I'm working to have this added. I'll have to check with the lab about the peptide for the SIRT7...

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