Anti-SIT antibody [SIT-01] (ab9528)


  • Product nameAnti-SIT antibody [SIT-01]
    See all SIT primary antibodies
  • Description
    Mouse monoclonal [SIT-01] to SIT
  • SpecificityHuman SHP2-interacting transmembrane adapter protein (SIT).
  • Tested applicationsSuitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant intracellular fragment (Human).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by protein-A affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberSIT-01
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab9528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionNegatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.
  • Tissue specificitySpecifically expressed in T- and B-cells. Present in plasma cells but not in germinal center B-cells (at protein level). Expressed in T- and B-cell lymphoma.
  • Post-translational
    Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • gp30/40 antibody
    • SHP intracting transmembrane adapter protein antibody
    • SHP2-interacting transmembrane adapter protein antibody
    • Signaling threshold-regulating transmembrane adapter 1 antibody
    • SIT antibody
    • Sit1 antibody
    • SIT1_HUMAN antibody
    • Suppression inducing transmembrane adapter 1 antibody
    • Suppression-inducing transmembrane adapter 1 antibody
    see all

Anti-SIT antibody [SIT-01] images

  • IHC image of ab9528 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9528, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-SIT antibody [SIT-01] (ab9528)

ab9528 has not yet been referenced specifically in any publications.

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