Anti-SIV1 Nef antibody [17] (ab42361)

Overview

  • Product name
    Anti-SIV1 Nef antibody [17]
  • Description
    Mouse monoclonal [17] to SIV1 Nef
  • Specificity
    Reacts with SIV of mac251 strain.
  • Tested applications
    Suitable for: ELISA, WB, Radioimmunoprecipitation, ICC/IFmore details
  • Species reactivity
    Simian Immunodeficiency Virus 1.
  • Immunogen

    Recombinant Nef protein of SIV1 mac251 strain.

  • Epitope
    Mapped to amino acids 71-80 (PAEEREKLAY).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified by affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    17
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/1000.
Radioimmunoprecipitation 1/5000.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    The Nef proteins of HIV-1 and SIV are 27- to 32 -kDa myristoylated proteins that are found in both the membranes and cytosol of infected host cells. While not strictly required for viral infection and replication, attenuation of Nef expression in either HIV-1 or SIV is associated with markedly delayed progression of disease. Simian immunodeficiency virus (SIV) and HIV-1 Nef proteins are related regulatory proteins that share several functions, including the ability to downregulate class I major histocompatibility complex (MHC) and CD4 expression on the cell surface and to alter T-cell-receptor-initiated signal transduction in T cells. Nef is thus probably involved in viral immune evasion mechanisms.
  • Cellular localization
    Cell Membrane, Lipid-anchor.
  • Alternative names
    • 3'ORF antibody
    • F protein antibody
    • Negative factor antibody

References

ab42361 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

For ab42361: DISCOUNT CODE: ***

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Expiration date: August 5th, 2012

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Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (PBMC)
Loading amount
25 µg
Specification
PBMC
Treatment
SIV-infected
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Karppiah Muthumani

Verified customer

Submitted Jun 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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