Anti-SIV1 Nef antibody [17] (ab42361)

Overview

  • Product nameAnti-SIV1 Nef antibody [17]
  • Description
    Mouse monoclonal [17] to SIV1 Nef
  • SpecificityReacts with SIV of mac251 strain.
  • Tested applicationsSuitable for: ELISA, WB, Radioimmunoprecipitation, ICC/IFmore details
  • Species reactivity
    Simian Immunodeficiency Virus 1.
  • Immunogen

    Recombinant Nef protein of SIV1 mac251 strain.

  • EpitopeMapped to amino acids 71-80 (PAEEREKLAY).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified by affinity chromatography.
  • ClonalityMonoclonal
  • Clone number17
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/1000.
Radioimmunoprecipitation 1/5000.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceThe Nef proteins of HIV-1 and SIV are 27- to 32 -kDa myristoylated proteins that are found in both the membranes and cytosol of infected host cells. While not strictly required for viral infection and replication, attenuation of Nef expression in either HIV-1 or SIV is associated with markedly delayed progression of disease. Simian immunodeficiency virus (SIV) and HIV-1 Nef proteins are related regulatory proteins that share several functions, including the ability to downregulate class I major histocompatibility complex (MHC) and CD4 expression on the cell surface and to alter T-cell-receptor-initiated signal transduction in T cells. Nef is thus probably involved in viral immune evasion mechanisms.
  • Cellular localizationCell Membrane, Lipid-anchor.
  • Alternative names
    • 3'ORF antibody
    • F protein antibody
    • Negative factor antibody

References for Anti-SIV1 Nef antibody [17] (ab42361)

ab42361 has not yet been referenced specifically in any publications.

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Expiration date: August 5th, 2012

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Application Western blot
Sample African Green Monkey Cell lysate - whole cell (PBMC)
Loading amount 25 µg
Specification PBMC
Treatment SIV-infected
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Karppiah Muthumani

Verified customer

Submitted Jun 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"