SIVA overexpression 293T lysate (whole cell) (ab94200)

Overview

  • Product nameSIVA overexpression 293T lysate (whole cell)
    See all SIVA lysates
  • Description
    SIVA overexpression lysate
  • General notesab94200 is a 293T cell transfected lysate in which Human SIVA has been transiently over-expressed using a pCMV-SIVA plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundFunction: Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl-x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis. Tissue specificity: Ubiquitous. Mostly expressed in thymus, testis, ovary, prostate, small intestine and spleen and less in colon. PTM: Phosphorylated by ABL2/ARG in response to oxidative stress.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

SIVA overexpression 293T lysate (whole cell) images

  • ab94200 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-SIVA antibody (ab67620) at 1/500 dilution

    Lane 1 : SIVA overexpression 293T lysate (whole cell) (ab94200)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjug at 1/2500 dilution

References for SIVA overexpression 293T lysate (whole cell) (ab94200)

ab94200 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"