Overview

  • Product nameAnti-SIX homeobox 4 antibody
    See all SIX homeobox 4 primary antibodies
  • Description
    Rabbit polyclonal to SIX homeobox 4
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Dog
  • Immunogen

    Synthetic peptide designed within residues: SSSSPTGQIA SAADIKQENG MESASEGQEA HREVAGGAAV GLSPPAPAPF, corresponding to N-terminal amino acids 2 - 51 of Human SIX homeobox 4 (NP_059116)

  • Positive control
    • HeLa cell lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-SIX homeobox 4 antibody images

  • ab87242 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87242 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-SIX homeobox 4 antibody (ab87242) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 83 kDa

References for Anti-SIX homeobox 4 antibody (ab87242)

ab87242 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"