Overview

  • Product nameAnti-SIX1 antibody
    See all SIX1 primary antibodies
  • Description
    Rabbit polyclonal to SIX1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Three synthetic peptides (human) conjugated to KLH

  • Positive control
    • An induced culture of E. coli

Properties

Applications

Our Abpromise guarantee covers the use of ab86028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 32 kDa). Tag is 14kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay be involved in limb tendon and ligament development.
    • Tissue specificitySpecifically expressed in skeletal muscle.
    • Involvement in diseaseDefects in SIX1 are the cause of deafness autosomal dominant type 23 (DFNA23) [MIM:605192]. A form of non-syndromic deafness characterized by prelingual, bilateral, symmetric hearing loss with a conductive component present in some but not all patients.
      Defects in SIX1 are the cause of branchiootic syndrome type 3 (BOS3) [MIM:608389]. BOS3 is a syndrome characterized by usually bilateral branchial cleft fistulas or cysts, sensorineural and/or conductive hearing loss, pre-auricular pits, and structural defects of the outer, middle or inner ear. Otic defects include malformed and hypoplastic pinnae, a narrowed external ear canal, bulbous internal auditory canal, stapes fixation, malformed and hypoplastic cochlea. Branchial and otic anomalies are as those seen in individuals with the branchiootorenal syndrome. However, renal anomalies are absent in branchiootic syndrome patients.
      Note=Defects in SIX1 could be a cause of branchiootorenal syndrome (BOR). BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable.
    • Sequence similaritiesBelongs to the SIX/Sine oculis homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BOS3 antibody
      • DFNA23 antibody
      • Homeobox protein SIX1 antibody
      • OTTHUMP00000179042 antibody
      • Sine oculis homeobox homolog 1 antibody
      • SIX homeobox 1 antibody
      • SIX1 antibody
      • SIX1_HUMAN antibody
      • TIP39 antibody
      see all

    Anti-SIX1 antibody images

    • All lanes : Anti-SIX1 antibody (ab86028) at 1/1000 dilution

      Lane 1 : An un-induced culture of E. coli
      Lane 2 : An induced culture of E. coli

      Lysates/proteins at 1/5000 dilution per lane.

      Secondary
      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 32 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 60 minutes

    References for Anti-SIX1 antibody (ab86028)

    ab86028 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"