Overview

  • Product nameAnti-Six5 antibody
    See all Six5 primary antibodies
  • Description
    Rabbit polyclonal to Six5
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Six5 aa 257-286 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    APCKSES DGNPTTEDES SRSPEDLERG AAPV

  • Positive control
    • MDA-MB231 cell line lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab113064 was purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ICC/IF 1/10 - 1/50.
WB 1/1000. Predicted molecular weight: 75 kDa.

Target

  • FunctionTranscription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter (By similarity). Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3.
  • Tissue specificityExpressed in adult but not in fetal eyes. Found in corneal epithelium and endothelium, lens epithelium, ciliary body epithelia, cellular layers of the retina and the sclera.
  • Involvement in diseaseDefects in SIX5 are the cause of branchiootorenal syndrome type 2 (BOR2) [MIM:610896]. BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable.
  • Sequence similaritiesBelongs to the SIX/Sine oculis homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageAt the begin of fourth week of development detected in cytoplasm of somite cells, and at the end of fourth week is accumulated in the nucleus. Between the sixth and eighth week of development detected in the nucleus of limb bud cells.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BOR2 antibody
    • DM locus associated homeodomain protein antibody
    • DM locus-associated homeodomain protein antibody
    • DMAHP antibody
    • Dystrophia myotonica associated homeodomain protein antibody
    • Homeobox protein SIX5 antibody
    • Sine oculis homeobox homolog 5 antibody
    • sine oculis related homeobox 5 homolog (Drosophila) antibody
    • SIX homeobox 5 antibody
    • SIX5 antibody
    • SIX5_HUMAN antibody
    see all

Anti-Six5 antibody images

  • Immunohistochemistry of formalin fixed and paraffin embedded Human liver tissue labelling Six5 with ab113064.

  • Immunofluorescence of HeLa cells labelling Six5 with ab113064. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab113064 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Six5 immunoreactivity is localized to the cytoplasm.

  • Anti-Six5 antibody (ab113064) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg

    Predicted band size : 75 kDa

References for Anti-Six5 antibody (ab113064)

ab113064 has not yet been referenced specifically in any publications.

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