• Product nameAnti-Six5 antibody
    See all Six5 primary antibodies
  • Description
    Rabbit polyclonal to Six5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of human Six5.

  • Positive control
    • extracts from K562 cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab74071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ELISA 1/40000.


  • FunctionTranscription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter (By similarity). Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3.
  • Tissue specificityExpressed in adult but not in fetal eyes. Found in corneal epithelium and endothelium, lens epithelium, ciliary body epithelia, cellular layers of the retina and the sclera.
  • Involvement in diseaseDefects in SIX5 are the cause of branchiootorenal syndrome type 2 (BOR2) [MIM:610896]. BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable.
  • Sequence similaritiesBelongs to the SIX/Sine oculis homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageAt the begin of fourth week of development detected in cytoplasm of somite cells, and at the end of fourth week is accumulated in the nucleus. Between the sixth and eighth week of development detected in the nucleus of limb bud cells.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BOR2 antibody
    • DM locus associated homeodomain protein antibody
    • DM locus-associated homeodomain protein antibody
    • DMAHP antibody
    • Dystrophia myotonica associated homeodomain protein antibody
    • Homeobox protein SIX5 antibody
    • Sine oculis homeobox homolog 5 antibody
    • sine oculis related homeobox 5 homolog (Drosophila) antibody
    • SIX homeobox 5 antibody
    • SIX5 antibody
    • SIX5_HUMAN antibody
    see all

Anti-Six5 antibody images

  • All lanes : Anti-Six5 antibody (ab74071) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from K562 cells with immunizing peptide at 10 µg

    Lysates/proteins at 20 µg per lane.

    Predicted band size : 75 kDa
    Observed band size : 75 kDa

References for Anti-Six5 antibody (ab74071)

ab74071 has not yet been referenced specifically in any publications.

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