Overview

  • Product nameAnti-SKA1 antibody
    See all SKA1 primary antibodies
  • Description
    Rabbit polyclonal to SKA1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, derived from the C-terminus of Human SKA1.

  • Positive control
    • A549 cell lysate; Human Brain, Cerebellum and Cortex tissues

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 29 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionComponent of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.
  • Sequence similaritiesBelongs to the SKA1 family.
  • Cellular localizationCytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins. Associates with kinetochores following microtubule attachment from prometaphase, through mid-anaphase and then vanishes in telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810433K01Rik antibody
    • AV117428 antibody
    • C18orf24 antibody
    • MGC10200 antibody
    • Ska1 antibody
    • SKA1_HUMAN antibody
    • Spindle and kinetochore associated complex subunit 1 antibody
    • Spindle and kinetochore associated protein 1 antibody
    • Spindle and kinetochore-associated protein 1 antibody
    • Spindle and KT (kinetochore) associated 1 antibody
    • Spindle and KT associated 1 antibody
    see all

Anti-SKA1 antibody images

  • Lane 1 : Anti-SKA1 antibody (ab118586) at 0.5 µg/ml
    Lane 2 : Anti-SKA1 antibody (ab118586) at 1 µg/ml

    Lane 1 : A549 cell lysate
    Lane 2 : A549 cell lysate


    Predicted band size : 29 kDa
  • ab118586, at 5 µg/ml, staining SKA1 in formalin-fixed, paraffin-embedded Human Brain, Cerebellum tissue by Immunohistochemistry.
  • ab118586, at 5 µg/ml, staining SKA1 in formalin-fixed, paraffin-embedded Human Brain, Cortex tissue by Immunohistochemistry.

References for Anti-SKA1 antibody (ab118586)

ab118586 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"