Overview

  • Product nameAnti-SKA1 antibody
    See all SKA1 primary antibodies
  • Description
    Rabbit polyclonal to SKA1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Cow, Cat, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 51-100 (LLNKFELEIQ YQEQTNSSLK ELCESLREEC EDVEHLKEHV PPHLPQVTAT) of Mouse SKA1 (NP_079857).

  • Positive control
    • Mouse pancreas lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab125419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionComponent of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.
  • Sequence similaritiesBelongs to the SKA1 family.
  • Cellular localizationCytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins. Associates with kinetochores following microtubule attachment from prometaphase, through mid-anaphase and then vanishes in telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810433K01Rik antibody
    • AV117428 antibody
    • C18orf24 antibody
    • MGC10200 antibody
    • Ska1 antibody
    • SKA1_HUMAN antibody
    • Spindle and kinetochore associated complex subunit 1 antibody
    • Spindle and kinetochore associated protein 1 antibody
    • Spindle and kinetochore-associated protein 1 antibody
    • Spindle and KT (kinetochore) associated 1 antibody
    • Spindle and KT associated 1 antibody
    see all

Anti-SKA1 antibody images

  • Anti-SKA1 antibody (ab125419) at 1 µg/ml + Mouse pancreas lysate at 10 µg

    Predicted band size : 29 kDa

References for Anti-SKA1 antibody (ab125419)

ab125419 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"