Overview

  • Product name
  • Description
    Rabbit polyclonal to SKA2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within N terminal amino acids 16-45 of Human SKA2 (NP_872426.1).

  • Positive control
    • T47D cell line lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab113067 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 14 kDa.

Target

  • Function
    Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization.
  • Sequence similarities
    Belongs to the SKA2 family.
  • Cellular localization
    Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • FAM33A antibody
    • Family with sequence similarity 33, member A antibody
    • FLJ12758 antibody
    • MGC110975 antibody
    • Protein FAM33A antibody
    • SKA 2 antibody
    • SKA2 antibody
    • SKA2_HUMAN antibody
    • Spindle and kinetochore associated complex subunit 2 antibody
    • Spindle and kinetochore associated protein 2 antibody
    • Spindle and kinetochore-associated protein 2 antibody
    • Spindle and KT (kinetochore) associated 2 antibody
    • Spindle and KT associated 2 antibody
    see all

Images

  • Anti-SKA2 antibody (ab113067) at 1/100 dilution + T47D cell line lysate at 35 µg

    Predicted band size : 14 kDa

References

ab113067 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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I am sorry about the problems you are having with ab113067 in western blot. As we discussed on the phone, I am sending ab14899, T47D cell lysate for you to use as a positive control. Your order numbe...

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