Overview

  • Product name
  • Description
    Rabbit polyclonal to SKA2
  • Specificity
    This antibody is specific for SKA2 and will not crossreact with SKA1 or SKA3.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 15 amino acid peptide from near the N terminus of Human SKA2 (NP_872426).

  • Positive control
    • 3T3 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity chromatography purified via peptide column
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 14 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization.
    • Sequence similarities
      Belongs to the SKA2 family.
    • Cellular localization
      Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins.
    • Information by UniProt
    • Database links
    • Alternative names
      • FAM33A antibody
      • Family with sequence similarity 33, member A antibody
      • FLJ12758 antibody
      • MGC110975 antibody
      • Protein FAM33A antibody
      • SKA 2 antibody
      • SKA2 antibody
      • SKA2_HUMAN antibody
      • Spindle and kinetochore associated complex subunit 2 antibody
      • Spindle and kinetochore associated protein 2 antibody
      • Spindle and kinetochore-associated protein 2 antibody
      • Spindle and KT (kinetochore) associated 2 antibody
      • Spindle and KT associated 2 antibody
      see all

    Anti-SKA2 antibody images

    • Lane 1 : Anti-SKA2 antibody (ab91551) at 0.5 µg/ml
      Lane 2 : Anti-SKA2 antibody (ab91551) at 1 µg/ml

      Lane 1 : 3T3 cell lysate
      Lane 2 : 3T3 cell lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 14 kDa

    References for Anti-SKA2 antibody (ab91551)

    ab91551 has not yet been referenced specifically in any publications.

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