Overview

  • Product nameAnti-SKA2 antibody
    See all SKA2 primary antibodies
  • Description
    Rabbit polyclonal to SKA2
  • SpecificityThis antibody is specific for SKA2 and will not crossreact with SKA1 or SKA3.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 15 amino acid peptide from near the N terminus of Human SKA2 (NP_872426).

  • Positive control
    • 3T3 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity chromatography purified via peptide column
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab91551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 14 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization.
    • Sequence similaritiesBelongs to the SKA2 family.
    • Cellular localizationCytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins.
    • Information by UniProt
    • Database links
    • Alternative names
      • FAM33A antibody
      • Family with sequence similarity 33, member A antibody
      • FLJ12758 antibody
      • MGC110975 antibody
      • Protein FAM33A antibody
      • SKA 2 antibody
      • SKA2 antibody
      • SKA2_HUMAN antibody
      • Spindle and kinetochore associated complex subunit 2 antibody
      • Spindle and kinetochore associated protein 2 antibody
      • Spindle and kinetochore-associated protein 2 antibody
      • Spindle and KT (kinetochore) associated 2 antibody
      • Spindle and KT associated 2 antibody
      see all

    Anti-SKA2 antibody images

    • Lane 1 : Anti-SKA2 antibody (ab91551) at 0.5 µg/ml
      Lane 2 : Anti-SKA2 antibody (ab91551) at 1 µg/ml

      Lane 1 : 3T3 cell lysate
      Lane 2 : 3T3 cell lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 14 kDa

    References for Anti-SKA2 antibody (ab91551)

    ab91551 has not yet been referenced specifically in any publications.

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