Overview

  • Product nameAnti-SKA3 antibody
    See all SKA3 primary antibodies
  • Description
    Rabbit polyclonal to SKA3
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, from the C-terminal region of Human SKA3.

  • Positive control
    • Human testis tissue; Human testis tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 98.98% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 46 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionComponent of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.
  • Sequence similaritiesBelongs to the SKA3 family.
  • Cellular localizationCytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • C13orf3 antibody
    • RAMA1 antibody
    • SKA3 antibody
    • SKA3_HUMAN antibody
    • Spindle and kinetochore associated complex subunit 3 antibody
    • Spindle and kinetochore-associated protein 3 antibody
    see all

Anti-SKA3 antibody images

  • ab118560 at 5µg/ml staining SKA3 in Formalin-fixed, Paraffin-embedded Human testis tissue by immunohistochemistry.
  • Lane 1 : Anti-SKA3 antibody (ab118560) at 0.5 µg/ml
    Lane 2 : Anti-SKA3 antibody (ab118560) at 1 µg/ml

    Lane 1 : Human testis tissue lysate
    Lane 2 : Human testis tissue lysate


    Predicted band size : 46 kDa

References for Anti-SKA3 antibody (ab118560)

ab118560 has not yet been referenced specifically in any publications.

Product Wall

Thank you for keeping me updated and sending the images of the results.

It does look like the results are a little cleaner, though I'm sorry to see that the results are still not as expected. I believe that the two bands in the testis lysate ...

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Thank you so much for your reply and for sending the images.

The images will be very useful for our investigation into the trouble with these antibodies. Please keep me updated about the results with the testis lysate, and let me know if ther...

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Thank you again for your call today.

I've heard back from the lab, and the Western blot protocol used to test ab118560 is attached to this email.

Please keep me updated about the results using the positive control testis lysate, and...

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Thank you for your call today and for letting us know about the trouble with ab118560. I apologize for the trouble with our SKA3 antibodies.

As we discussed, I'm sending a free of charge vial of ab30257 human testis lysate to use as a positiv...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"