Overview

  • Product nameAnti-SKA3 antibody
    See all SKA3 primary antibodies
  • Description
    Rabbit polyclonal to SKA3
  • Specificityab91559 is specific for SKA3 and will not crossreact with SKA1 or SKA2.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 13 amino acid peptide from near the C terminal of Human SKA3 (NP_659498).

  • Positive control
    • Human testis tissue lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab91559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 46 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.
    • Sequence similaritiesBelongs to the SKA3 family.
    • Cellular localizationCytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner.
    • Information by UniProt
    • Database links
    • Alternative names
      • C13orf3 antibody
      • RAMA1 antibody
      • SKA3 antibody
      • SKA3_HUMAN antibody
      • Spindle and kinetochore associated complex subunit 3 antibody
      • Spindle and kinetochore-associated protein 3 antibody
      see all

    Anti-SKA3 antibody images

    • Lane 1 : Anti-SKA3 antibody (ab91559) at 0.5 µg/ml
      Lane 2 : Anti-SKA3 antibody (ab91559) at 1 µg/ml

      Lane 1 : Human testis tissue lysate
      Lane 2 : Human testis tissue lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 46 kDa

    References for Anti-SKA3 antibody (ab91559)

    ab91559 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for keeping me updated and sending the images of the results.

    It does look like the results are a little cleaner, though I'm sorry to see that the results are still not as expected. I believe that the two bands in the testis lysate ...

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    Thank you so much for your reply and for sending the images.

    The images will be very useful for our investigation into the trouble with these antibodies. Please keep me updated about the results with the testis lysate, and let me know if ther...

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    Thank you for your call today and for letting us know about the trouble with ab118560. I apologize for the trouble with our SKA3 antibodies.

    As we discussed, I'm sending a free of charge vial of ab30257 human testis lysate to use as a positiv...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"