Overview

  • Product nameAnti-SKAP55 antibody
    See all SKAP55 primary antibodies
  • Description
    Mouse monoclonal to SKAP55
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-359 of Human SKAP55

Properties

Applications

Our Abpromise guarantee covers the use of ab58316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPositively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
    • Tissue specificityHighly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level).
    • Sequence similaritiesBelongs to the SKAP family.
      Contains 1 PH domain.
      Contains 1 SH3 domain.
    • DomainThe SH3 domain interacts with FYB.
    • Post-translational
      modifications
      Phosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC.
    • Cellular localizationCytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • pp55 antibody
      • SCAP 1 antibody
      • SCAP1 antibody
      • SKAP 1 antibody
      • SKAP 55 antibody
      • SKAP-55 antibody
      • Skap1 antibody
      • SKAP1_HUMAN antibody
      • SKAP55 adaptor protein antibody
      • SRC family associated phosphoprotein 1 antibody
      • Src family-associated phosphoprotein 1 antibody
      • Src kinase associated phosphoprotein 1 antibody
      • SRC kinase associated phosphoprotein 55 kD antibody
      • Src kinase associated phosphoprotein of 55 kDa antibody
      • Src kinase-associated phosphoprotein 1 antibody
      • Src kinase-associated phosphoprotein of 55 kDa antibody
      see all

    Anti-SKAP55 antibody images

    • SKAP55 antibody (ab58316) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
    • Western blot against tagged recombinant protein immunogen using ab58316 SKAP55 antibody at 1ug/ml. Predicted band size of immunogen is 65 kDa

    References for Anti-SKAP55 antibody (ab58316)

    ab58316 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"