Recombinant
RabMAb

Anti-SKAP55 antibody [EPR11360] (ab170882)

Overview

  • Product name
    Anti-SKAP55 antibody [EPR11360]
    See all SKAP55 primary antibodies
  • Description
    Rabbit monoclonal [EPR11360] to SKAP55
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SKAP55 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q86WV1

  • Positive control
    • Human thymus, Jurkat, HuT-78 and MOLT4 lysates, Jurkat cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
    • Tissue specificity
      Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level).
    • Sequence similarities
      Belongs to the SKAP family.
      Contains 1 PH domain.
      Contains 1 SH3 domain.
    • Domain
      The SH3 domain interacts with FYB.
    • Post-translational
      modifications
      Phosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC.
    • Cellular localization
      Cytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • pp55 antibody
      • SCAP 1 antibody
      • SCAP1 antibody
      • SKAP 1 antibody
      • SKAP 55 antibody
      • SKAP-55 antibody
      • Skap1 antibody
      • SKAP1_HUMAN antibody
      • SKAP55 adaptor protein antibody
      • SRC family associated phosphoprotein 1 antibody
      • Src family-associated phosphoprotein 1 antibody
      • Src kinase associated phosphoprotein 1 antibody
      • SRC kinase associated phosphoprotein 55 kD antibody
      • Src kinase associated phosphoprotein of 55 kDa antibody
      • Src kinase-associated phosphoprotein 1 antibody
      • Src kinase-associated phosphoprotein of 55 kDa antibody
      see all

    Images

    • All lanes : Anti-SKAP55 antibody [EPR11360] (ab170882) at 1/1000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : Jurkat lysate
      Lane 3 : HuT-78 lysate
      Lane 4 : MOLT4 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 41 kDa

    • Immunofluorescent staining of Jurkat cells labeling SKAP55 using ab170882 at a 1/100 dilution (green) and DAPI nuclear staining (blue).

    • Flow cytometric analysis of permeabilized Jurkat cells labeling SKAP55 using ab170882 at a 1/10 dilution (red) or a rabbit IgG (green).

    References

    ab170882 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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