Overview

  • Product name
  • Description
    Rabbit polyclonal to SKAR
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region of Human SKAR.

  • Positive control
    • 721 B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • 46 kDa DNA polymerase delta interaction protein antibody
    • DNA polymerase delta interacting protein 3 antibody
    • KIAA1649 antibody
    • P46 antibody
    • PDIP3_HUMAN antibody
    • PDIP46 antibody
    • POLDIP3 antibody
    • Polymerase (DNA-directed), delta interacting protein 3 antibody
    • Polymerase delta interacting protein 46 antibody
    • Polymerase delta-interacting protein 3 antibody
    • RNA-binding protein P46 antibody
    • S6K1 Aly/REF-like target antibody
    • SKAR antibody
    see all

Images

  • Anti-SKAR antibody (ab80313) at 1 µg/ml + 721 B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ab80313 at 10 µg/ml staining SKAR in Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab80313 at 10 µg/ml staining SKAR in Human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab80313 at 10 µg/ml staining SKAR in Human small intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab80313 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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