Anti-Skeletrophin / Mib2 antibody (ab99378)

Overview

  • Product nameAnti-Skeletrophin / Mib2 antibody
    See all Skeletrophin / Mib2 primary antibodies
  • Description
    Rabbit polyclonal to Skeletrophin / Mib2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 925 and 975 of Human Skeletrophin/ Mib2 (NP_543151.1).

  • Positive control
    • HeLa, 293T lysates
  • General notesImmunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 110 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceSkeletrophin, also known as Mib2, is a homolog of the zebrafish protein Mindbomb. Skeletrophin is an E3 ubiquitin ligase, expressed mainly in skeletal muscle, which is downregulated in malignant melanoma.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase MIB2 antibody
    • Mib2 antibody
    • Mindbomb homolog 2 antibody
    • Novel zinc finger protein antibody
    • Novelzin antibody
    • Putative NF-kappa-B-activating protein 002N antibody
    • SKD antibody
    • Skeletrophin antibody
    • Zinc finger ZZ type with ankyrin repeat domain protein 1 antibody
    • ZZANK1 antibody
    see all

Anti-Skeletrophin / Mib2 antibody images

  • All lanes : Anti-Skeletrophin / Mib2 antibody (ab99378) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 110 kDa


    Exposure time : 30 seconds
  • Lane 1 : Detection of Human Skeletrophin / Mib2 by Imunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99378 at 3µg/mg lysate. Lane 2 : Control IgG

References for Anti-Skeletrophin / Mib2 antibody (ab99378)

ab99378 has not yet been referenced specifically in any publications.

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