Overview

  • Product nameAnti-Skp1 antibody
    See all Skp1 primary antibodies
  • Description
    Rabbit polyclonal to Skp1
  • SpecificityThis antibody detects a 19kD band in Hela and HEK293 cells. The band can be blocked with the immunising peptide. The antibody shows extensive staining (eg. of lymphoid and epithelial cells), with staining of nuclei and some staining of the cytoplasm, when tested on human tonsil in IHC.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Guinea pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide derived from residues 50 - 150 of Human Skp1.

  • Positive control
    • This antibody gave a positive signal in HeLa nuclear lysate as well as the following whole cell lysates: HeLa; HEK293. IF/ICC: MCF7 cell line

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab10546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 19 kDa (predicted molecular weight: 18.5 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionEssential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the SKP1 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cyclin A/CDK2 associated p19 antibody
    • Cyclin A/CDK2 associated protein p19 antibody
    • Cyclin-A/CDK2-associated protein p19 antibody
    • EMC19 antibody
    • MGC34403 antibody
    • OCP 2 antibody
    • OCP II antibody
    • OCP II protein antibody
    • OCP-2 antibody
    • OCP-II antibody
    • OCP2 antibody
    • OCPII antibody
    • Organ of Corti protein 2 antibody
    • Organ of Corti protein II antibody
    • OTTHUMP00000159379 antibody
    • OTTHUMP00000159380 antibody
    • OTTHUMP00000228275 antibody
    • OTTHUMP00000228276 antibody
    • OTTHUMP00000228278 antibody
    • OTTHUMP00000228279 antibody
    • OTTHUMP00000228281 antibody
    • p19A antibody
    • p19skp1 antibody
    • RNA polymerase II elongation factor like protein antibody
    • RNA polymerase II elongation factor like protein OCP2 antibody
    • RNA polymerase II elongation factor-like protein antibody
    • S phase kinase associated protein 1 antibody
    • S phase kinase associated protein 1A antibody
    • S phase kinase associated protein 1A p19A antibody
    • S-phase kinase-associated protein 1 antibody
    • SIII antibody
    • Skp 1 antibody
    • SKP 1A antibody
    • skp1 antibody
    • SKP1_HUMAN antibody
    • SKP1A antibody
    • TCEB1L antibody
    • Transcription Elongation Factor B 1 like antibody
    • Transcription elongation factor B antibody
    • Transcription elongation factor B SIII polypeptide 1 like antibody
    see all

Anti-Skp1 antibody images

  • ICC/IF image of ab10546 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10546, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18.5 kDa


    Exposure time : 2 minutes

    Western blot using ab10546 at 1/500.

    Lane 1: Hela nuclear
    Lane 2: Hela
    Lane 3: HEK293
    Lane 4: Hela nuclear + ab10546 blocking peptide (1µg/ml)
    Lane 5: Hela + ab10546 blocking peptide (1µg/ml)
    Lane 6: HEK293 + ab10546 blocking peptide (1µg/ml)

    Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
    Exposure time: 2 mins.

  • ab10546 was used in immunohistochemistry with paraffin embedded sections of human tonsil, using DAB as a chromogen (brown). Counterstaining of nuclei was performed with haemotoxylin (blue).
    Strong staining of epithelial cell nuclei is seen (and some staining of the cytoplasm) in this image.

     

     


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18.5 kDa


    Exposure time : 20 seconds

References for Anti-Skp1 antibody (ab10546)

This product has been referenced in:
  • Bruckert WM  et al. Rapid nutritional remodeling of the host cell upon attachment of Legionella pneumophila. Infect Immun 82:72-82 (2014). Read more (PubMed: 24126522) »
  • Viñas-Castells R  et al. Nuclear ubiquitination by FBXL5 modulates Snail1 DNA binding and stability. Nucleic Acids Res N/A:N/A (2013). WB . Read more (PubMed: 24157836) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample Dog Tissue sections (MDCK pellets in paraffin)
Specification MDCK pellets in paraffin
Permeabilization Yes - Tween-20
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 04 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (HEK293T)
Specification HEK293T
Treatment 100 ´M FAC (for 6 h) and 10 ´M MG132 (for 4 h)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 08 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Nov 30 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"