The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: 1/50.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
WB: 1/1000 - 1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Is unsuitable for ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionEssential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
PathwayProtein modification; protein ubiquitination.
ab80586 at 1/50 dilution (red) staining Skp1 in permeabilized HeLa cells by Flow cytometry. Rabbit IgG (negative) is shown in green.
References for Anti-Skp1 antibody [EPR3303] (ab80586)
This product has been referenced in:
Wang D et al. Inhibition of S-phase kinase-associated protein 2 (Skp2) reprograms and converts diabetogenic T cells to Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A109:9493-8 (2012).
Read more (PubMed: 22645357) »